AR51630PU-N glk (1-321, His-tag)

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0.5 mg / €730.00
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glk

Product Description for glk

glk.
Presentation: Purified

Properties for glk

Product Category Proteins & Growth Factors
Quantity 0.5 mg
Synonyms ECK2384, Glucokinase, JW2385
Presentation Purified
Molecular weight 37.1 kDa
Source E. coli
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P0A6V8
Purity > 95 % by SDS - PAGE
Background glk belongs to the bacterial glucokinase family. This protein is not highly important in E.coli as glucose is transported into the cell by the PTS system already as glucose 6-phosphate.
Concentration 1.0 mg/ml (determined by Bradford assay)
General Readings Kawai S, Mukai T, et al. (2005) J. Biosci. Bioeng. 99 (4): 320-30.
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description
Description:
Recombinant E.coli glk protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGSMTKYALV GDVGGTNARL ALCDIASGEI SQAKTYSGLD YPSLEAVIRV YLEEHKVEVK DGCIAIACPI TGDWVAMTNH TWAFSIAEMK KNLGFSHLEI INDFTAVSMA IPMLKKEHLI QFGGAEPVEG KPIAVYGAGT GLGVAHLVHV DKRWVSLPGE GGHVDFAPNS EEEAIILEIL RAEIGHVSAE RVLSGPGLVN LYRAIVKADN RLPENLKPKD ITERALADSC TDCRRALSLF CVIMGRFGGN LALNLGTFGG VFIAGGIVPR FLEFFKASGF RAAFEDKGRF KEYVHDIPVY LIVHDNPGLL GSGAHLRQTL GHIL
Molecular weight:
(344aa) confirmed by MALDI-TOF
Format
Buffer System:
20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol
Purity:
>95% by SDS - PAGE
State:
Liquid purified protein
Purified
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