AR09993PU-L Glutaminase 1 / glsA1 (1-310, His-tag)

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0.5 mg / €750.00
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Glutaminase 1 / glsA1

AR09993PU-L

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  • AR09993PU-L

Product Description for Glutaminase 1 / glsA1

Glutaminase 1 / glsA1.
Presentation: Purified

Properties for Glutaminase 1 / glsA1

Product Category Proteins & Growth Factors
Quantity 0.5 mg
Synonyms JW0474, b0485, ybaS
Presentation Purified
Molecular weight kDa
Source E. coli
Species (Protein) E. coli
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P77454
Purity > 95 %
Background GlsA1 (glutaminase1) belongs to the large superfamily of serine-dependent beta-lactamases and penicillin-binding proteins. They are present in most bacteria and eukaryotes and catalyze the hydrolytic deamidation of l-glutamine to l-glutamate and free NH4+.
Concentration 1.0 mg/ml (determined by Bradford assay)
General Readings Brown G., et al. (2008) Biochemistry. 47(21):5724-35.
Nandakumar R., et al. (2003) J. Mol. Catal. B: Enzymes. 23:87–100.
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant E.coli glsA1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MLDANKLQQA VDQAYTQFHS LNGGQNADYI PFLANVPGQL AAVAIVTCDG NVYSAGDSDY RFALESISKV CTLALALEDV GPQAVQDKIG ADPTGLPFNS VIALELHGGK PLSPLVNAGA IATTSLINAE NVEQRWQRIL HIQQQLAGEQ VALSDEVNQS EQTTNFHNRA IAWLLYSAGY LYCDAMEACD VYTRQCSTLL NTIELATLGA TLAAGGVNPL THKRVLQADN VPYILAEMMM EGLYGRSGDW AYRVGLPGKS GVGGGILAVV PGVMGIAAFS PPLDEDGNSV RGQKMVASVA KQLGYNVFKG
Molecular weight:
(330aa), confirmed by MALDI-TOF
Format
Buffer System:
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100mM NaCl
Purity:
>95%
State:
Liquid purified protein
Purified
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