discontinued

PA1258 Glycogen Phosphorylase

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5 µg / €110.00

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Glycogen Phosphorylase

PA1258

Product Description for Glycogen Phosphorylase

Glycogen Phosphorylase.
Presentation: Purified

Properties for Glycogen Phosphorylase

Product Category Proteins & Growth Factors
Quantity 5 µg
Presentation Purified
Molecular weight 97 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 85 % Greater than 85.0% as determined by:
(a) Analysis by RP-HPLC
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained
Application Immunoassays and Western blot.
Background Glycogen Phosphorylase is an enzyme that catalyzes the degradation of Glycogen in animals by releasing glucose-1-phosphate from the terminal alpha-1,4-glycosidic bond.
This enzyme exists in two forms: an active phosphorylated form (Phosphorylase A) and an inactive un-phosphorylated form (Phosphorylase B).
Both a and b forms of phosphorylase exist as homodimers. In mammals, the major isozymes of glycogen phosphorylase are found in muscle, liver and brain tissue. Glycogen Phosphorylase- a sensitive marker for the AMI diagnosis within 4 hours after the onset of chest pain. It has also been shown that GPBB is increased in a considerable proportion of AMI patients within 2-3 hours from chest pain onset. GPBB is increased early in patients with unstable angina. Glycogen Phosphorylase can also be used as a sensitive marker for the detection of peri-operative myocardial ischaemia and infarction in patients undergoing coronary artery bypass grafting.
Concentration 0.8 mg/ml
General Readings
  1. Yu LJ, Chen Y, Treadway JL, McPherson RK, McCoid SC, Gibbs EM, et al. Establishment of correlation between in vitro enzyme binding potency and in vivo pharmacological activity: application to liver glycogen phosphorylase a inhibitors. J Pharmacol Exp Ther. 2006 Jun;317(3):1230-7. Epub 2006 Mar 14. PubMed PMID: 16537796.
  2. Jakobs S, Fridrich D, Hofem S, Pahlke G, Eisenbrand G. Natural flavonoids are potent inhibitors of glycogen phosphorylase. Mol Nutr Food Res. 2006 Jan;50(1):52-7. PubMed PMID: 16317787.
  3. Eronina TB, Chebotareva NA, Kurganov BI. Influence of osmolytes on inactivation and aggregation of muscle glycogen phosphorylase b by guanidine hydrochloride. Stimulation of protein aggregation under crowding conditions. Biochemistry (Mosc). 2005 Sep;70(9):1020-6. PubMed PMID: 16266274.
  4. Furukawa S, Murakami K, Nishikawa M, Nakayama O, Hino M. FR258900, a novel glycogen phosphorylase inhibitor isolated from Fungus No. 138354. II. Anti-hyperglycemic effects in diabetic animal models. J Antibiot (Tokyo). 2005 Aug;58(8):503-6. PubMed PMID: 16266121.
  5. FR258900, a novel glycogen phosphorylase inhibitor isolated from Fungus No. 138354. I. Taxonomy, fermentation, isolation and biological activities. J Antibiot (Tokyo) 2005 Aug;58(8):497-502
  6. Wen X, Zhang P, Liu J, Zhang L, Wu X, Ni P, et al. Pentacyclic triterpenes. Part 2: Synthesis and biological evaluation of maslinic acid derivatives as glycogen phosphorylase inhibitors. Bioorg Med Chem Lett. 2006 Feb;16(3):722-6. Epub 2005 Oct 21. PubMed PMID: 16246555.
Storage Recombinant Glycogen Phosphorylase, although stable at 10°C for 1 week, should be stored below -18°C.
Please avoid freeze-thaw cycles.
Shelf life: one year from despatch.
Source E. coli
Description
Description:
Recombinant Human Glycogen Phosphorylase produced in E. coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 97 kDa.Recombinant Glycogen Phosphorylase is purified by proprietary chromatographic techniques.
Format
Dimers:
Less than 1% as determined by silver-stained SDS-PAGE gel analysis
Purity:
>85% Greater than 85.0% as determined by:
(a) Analysis by RP-HPLC
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained
State:
Sterile filtered, colourless liquid. Each mg of protein contains 50% glycerol.
Purified
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