Product Description for gor
Properties for gor
|Product Category||Proteins & Growth Factors|
|Synonyms||ECK3485, Glutathione oxidoreductase, JW3467, gorA|
|Molecular weight||51.2 kDa|
|PDF datasheet||View Datasheet|
|Manufacturer||Acris Antibodies GmbH|
|Material safety datasheet||MSDS for Proteins (de)|
|Purity||> 90 % by SDS - PAGE|
|Background||gor, also known as Glutathione reductase, belongs to the class-I pyridine nucleotide disulfide oxidoreductase family. The main function of the protein is to maintain high levels of reduced glutathione in the cytosol. With the concomitant oxidation of NADPH, Glutathione reductase transforms oxidized glutathione to the reduced form. The active site of the protein is a redox-active disulfide bond.|
|Concentration||1.0 mg/ml (determined by Bradford assay)|
|Protocols||1. Prepare a 1,450 ul assay buffer. The final concentrations are 75mM Potassium phosphate (pH 7.5), 2.6mM EDTA, 1mM glutathione, 0.09mM beta-NADPH, and 0.13% BSA.
2. Add 50 ul of recombinant gor protein with 0.037 ug, 0.075 ug and 0.15 ug in assay buffer
3. Mix and load 200 ul of reaction mix in to a plate well.
4. Record the decrease in A340nm for 5 minutes at 25°C.
|General Readings||Staal G.E. et al. (1969) Biochim. Biophys. Acta 185: 63-69.Stoll V.S.. et al. (1997) Biochemistry 36: 6437-6447.|
|Storage||Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.