AR50248PU-S gor (1-450, His-tag)

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0.1 mg / €300.00
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gor

Product Description for gor

gor.
Presentation: Purified

Properties for gor

Product Category Proteins & Growth Factors
Quantity 0.1 mg
Synonyms ECK3485, Glutathione oxidoreductase, JW3467, gorA
Presentation Purified
Molecular weight 51.2 kDa
Source E. coli
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P06715
Purity > 90 % by SDS - PAGE
Background gor, also known as Glutathione reductase, belongs to the class-I pyridine nucleotide disulfide oxidoreductase family. The main function of the protein is to maintain high levels of reduced glutathione in the cytosol. With the concomitant oxidation of NADPH, Glutathione reductase transforms oxidized glutathione to the reduced form. The active site of the protein is a redox-active disulfide bond.
Concentration 1.0 mg/ml (determined by Bradford assay)
Protocols 1. Prepare a 1,450 ul assay buffer. The final concentrations are 75mM Potassium phosphate (pH 7.5), 2.6mM EDTA, 1mM glutathione, 0.09mM beta-NADPH, and 0.13% BSA.
2. Add 50 ul of recombinant gor protein with 0.037 ug, 0.075 ug and 0.15 ug in assay buffer
3. Mix and load 200 ul of reaction mix in to a plate well.
4. Record the decrease in A340nm for 5 minutes at 25°C.
General Readings Staal G.E. et al. (1969) Biochim. Biophys. Acta 185: 63-69.Stoll V.S.. et al. (1997) Biochemistry 36: 6437-6447.
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant E. coli gor protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGSMTKHYDY IAIGGGSGGI ASINRAAMYG QKCALIEAKE LGGTCVNVGC VPKKVMWHAA QIREAIHMYG PDYGFDTTIN KFNWETLIAS RTAYIDRIHT SYENVLGKNN VDVIKGFARF VDAKTLEVNG ETITADHILI ATGGRPSHPD IPGVEYGIDS DGFFALPALP ERVAVVGAGY IAVELAGVIN GLGAKTHLFV RKHAPLRSFD PMISETLVEV MNAEGPQLHT NAIPKAVVKN TDGSLTLELE DGRSETVDCL IWAIGREPAN DNINLEAAGV KTNEKGYIVV DKYQNTNIEG IYAVGDNTGA VELTPVAVAA GRRLSERLFN NKPDEHLDYS NIPTVVFSHP PIGTVGLTEP QAREQYGDDQ VKVYKSSFTA MYTAVTTHRQ PCRMKLVCVG SEEKIVGIHG IGFGMDEMLQ GFAVALKMGA TKKDFDNTVA IHPTAAEEFV TMR
Molecular weight:
(473aa) confirmed by MALDI-TOF
Specific activity:
> 52 unit/ml.
One unit will reduce 1.0 umol of oxidized glutathione per minute at pH 7.5 at 25°C.
Format
Buffer System:
20 mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 0.1M NaCl,1mM DTT
Purity:
>90% by SDS - PAGE
State:
Liquid purified protein
Purified
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