AR51496PU-S HCV NS5B (2634-2752, His-tag)

Search for all "HCV NS5B"

50 µg / €370.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

HCV NS5B

Product Description for HCV NS5B

HCV NS5B.
Presentation: Purified

Properties for HCV NS5B

Product Category Proteins & Growth Factors
Quantity 50 µg
Synonyms Hepatitis C Virus, RNA-directed RNA polymerase NS5B
Presentation Purified
Molecular weight 17.0 kDa
Source E. coli
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 90 % by SDS - PAGE
Background Hepatitis C virus genotype 1 is a core protein packages viral RNA to form a viral nucleocapsid, and promotes virion budding. It modulates viral translation initiation by interacting with HCV IRES and 40S ribosomal subunit and also regulates many host cellular functions such as signaling pathways and apoptosis. It prevents the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) and IFN-gamma signaling pathways and by inducing human STAT1 degradation. NS5B is a RNA-dependent RNA polymerase that plays an essential role in the virus replication.
Concentration 0.25 mg/ml (determined by Bradford assay)
General Readings
  1. Taylor DR, Tian B, Romano PR, Hinnebusch AG, Lai MM, Mathews MB. Hepatitis C virus envelope protein E2 does not inhibit PKR by simple competition with autophosphorylation sites in the RNA-binding domain. J Virol. 2001 Feb;75(3):1265-73. PubMed PMID: 11152499. (Free PMC Article available, 6 images available)
  2. Kalliampakou K.I., et al. (2015) J. Gen. Virol. 86:1015-1025
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant HCV (Hepatitis C Virus) NS5b protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSPMGF SYDTRCFDST VTESDIRTEE AIYQCCDLDP QARVAIKSLT ERLYVGGPLT NSRGENCGYR RCRASGVLTT SCGNTLTCYI KARAACRAAG LQDCTMLVCG DDLVVICESA GVQED
Molecular weight:
(155aa) confirmed by MALDI-TOF
Format
Buffer System:
20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Purity:
>90% by SDS - PAGE
State:
Liquid purified protein
Purified
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