discontinued

PA525X Heparin Binding EGF-like growth factor (HB-EGF)

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50 µg / €220.00

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Heparin Binding EGF-like growth factor (HB-EGF)

PA525X

Product Description for Heparin Binding EGF-like growth factor (HB-EGF)

Heparin Binding EGF-like growth factor (HB-EGF).

Properties for Heparin Binding EGF-like growth factor (HB-EGF)

Product Category Proteins & Growth Factors
Quantity 50 µg
Synonyms DTR, Diphteria toxin receptor
Molecular weight 9.7 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 95 %
Add. information Centrifuge the vial prior to opening.!
Background HB-EGF is an EGF related growth factor that signals through the EGF receptor, and stimulates the proliferation of smooth muscle cells (SMC), fibroblasts, epithelial cells, and keratinocytes. HB-EGF is expressed in numerous cell types and tissues, including vascular endothelial cells and SMC, macrophages, skeletal muscle, keratinocytes, and certain tumor cells. The ability of HB-EGF to specifically bind heparin and heparin sulfate proteoglycans is distinct from other EGF-like molecules, and may be related to the enhanced mitogenic activity, relative to EGF, that HB-EGF exerts on smooth muscle cells. The human HB-EGF gene encodes a 208 amino acid transmembrane protein, which can be proteolytically cleaved to produce soluble HB-EGF.
Storage Store the lyophilized protein at -20 °C.
Reconstituted human CTGF should be stored in working aliquots at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Source E. coli
Description
Description:
Recombinant human HB-EGF is a 9.7 kDa protein containing 86 amino acid residues, corresponding to the extra-cellular EGF-like and heparin binding domains of the full length HB-EGF protein.
AA Sequence:
DLQEADLDLL RVTLSSKPQA LATPNKEEHG KRKKKGKGLG KKRDPCLRKY KDFCIHGECK YVKELRAPS CICHPGYHGE RCHGLSL.
Biological activity:
The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is < 1.0 ng/ml.
Specific activity:
> 1 x 10e6 units/mg.
Format
Endotoxin level:
< 0.1 ng per μg (1 EU/μg)
Purity:
>95%
State:
Lyophilized purified fraction (>95% pure by SDS-PAGE gel and HPLC analyses) without preservatives.
Reconstitution:
Restore in water to a concentration of 0.1-1.0 mg/ml.
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