AR10549PU-L Hepatitis B Core /HBcAg (1-186)

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Hepatitis B Core /HBcAg


Product Description for Hepatitis B Core /HBcAg

Hepatitis B Core /HBcAg.
Presentation: Purified

Properties for Hepatitis B Core /HBcAg

Product Category Proteins & Growth Factors
Quantity 1 mg
Presentation Purified
Source E. coli
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity >90% pure as determined by 10% PAGE (coomassie staining).
Purification Method: Protein Precipitation.
Application Antigen in ELISA and Western blots, excellent antigen for detection of HBV with minimal specificity problems.
Background Hepatitis B is one of a few known non-retroviralviruses which employ reverse transcriptionas a part of its replication process. (HIV, a completely unrelated virus, also uses reverse transcription, but it is a retrovirus.) HBV invades the cell by binding to surface receptor and become internalized. The viral core particles then migrate to the hepatocyte nucleus and the partially double-stranded, relaxed circular genomes (RC-DNA) are repaired to form a covalently closed circular DNA (cccDNA), which is the template for viral genomic and sub-genomic RNAs by cellular RNA polymerase II. Of these, the pregenomic RNA(pgRNA is selectively packaged into progeny capsids and is then reverse-transcribed into new RC-DNA. The core can either bud into the endoplasmic reticulum to be enveloped or exported from the cell or recycled back into the genome for conversion to cccDNA. 
Concentration 1.0 mg/ml

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

The E.coli derived recombinant protein contains the HBV core immunodominant region, amino acids 1-186.
Specificity: Immunoreactive with sera of HBV-infected individuals.
>90% pure as determined by 10% PAGE (coomassie staining).
Purification Method: Protein Precipitation.
10mM Tris-HCl pH8.0, 50mM NaCl, 1mM EDTA, 50% Glycerol
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