Product Description for Hepatitis B Core /HBcAg
Properties for Hepatitis B Core /HBcAg
|Product Category||Proteins & Growth Factors|
|PDF datasheet||View Datasheet|
|Manufacturer||Acris Antibodies GmbH|
|Material safety datasheet||MSDS for Proteins (de)|
|Purity||>90% pure as determined by 10% PAGE (coomassie staining).
Purification Method: Protein Precipitation.
|Application||Antigen in ELISA and Western blots, excellent antigen for detection of HBV with minimal specificity problems.|
|Background||Hepatitis B is one of a few known non-retroviralviruses which employ reverse transcriptionas a part of its replication process. (HIV, a completely unrelated virus, also uses reverse transcription, but it is a retrovirus.) HBV invades the cell by binding to surface receptor and become internalized. The viral core particles then migrate to the hepatocyte nucleus and the partially double-stranded, relaxed circular genomes (RC-DNA) are repaired to form a covalently closed circular DNA (cccDNA), which is the template for viral genomic and sub-genomic RNAs by cellular RNA polymerase II. Of these, the pregenomic RNA(pgRNA is selectively packaged into progeny capsids and is then reverse-transcribed into new RC-DNA. The core can either bud into the endoplasmic reticulum to be enveloped or exported from the cell or recycled back into the genome for conversion to cccDNA.|
Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.