discontinued

AR10604PU-L Hepatitis C Virus Core, NS3, NS4, NS5

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1 mg / €570.00

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Hepatitis C Virus Core, NS3, NS4, NS5

AR10604PU-L

Product Description for Hepatitis C Virus Core, NS3, NS4, NS5

Hepatitis C Virus Core, NS3, NS4, NS5.
Presentation: Purified

Properties for Hepatitis C Virus Core, NS3, NS4, NS5

Product Category Proteins & Growth Factors
Quantity 1 mg
Presentation Purified
Source E. coli
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity >95.0% pure as determined by 10% PAGE (coomassie staining).
Purification Method: S-Sepharose > Ceramic Hydroxyapatite > Affinity Purification.
Application Antigen in ELISA and Western blots, excellent antigen for detection of HCV with minimal specificity problems.
Background HCV is a small 50nm, enveloped, single-stranded, positive sense RNAvirus in the family Flaviviridae.
HCV has a high rate of replication with approximately one trillion particles produced each day in an infected individual. Due to lack of proofreading by the HCV RNA polymerase, the HCV has an exceptionally high mutation rate, a factor that may help it elude the host's immune response. Hepatitis C virus is classified into six genotypes(1-6) with several subtypes within each genotype. The preponderance and distribution of HCV genotypes varies globally. Genotype is clinically important in determining potential response to interferon-based therapy and the required duration of such therapy. Genotypes 1 and 4 are less responsive to interferon-based treatment than are the other genotypes (2, 3, 5 and 6). 
Concentration 1.0 mg/ml
Storage

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Description
Description:
E.coli derived recombinant protein contains the HCV nucleocapsid, NS3, NS4, and NS5 immunodominant regions.
The E.Coli derived 70 kDa recombinant protein contains sequences from 4 gene products (proteins) of the hepatitis C virus (HCV) were scanned by using 3 different PCR-based techniques in search of the most immunoreactive regions suitable for the development of a diagnostic test for the detection of anti-HCV in human sera. All PCR fragments were cloned with pGEX4-2T expression vector and expressed in E. coli as chimeric proteins with glutathione S-transferase. The most diagnostically relevant proteins identified in this study were then contructed into one recombinant antigen. 
Specificity: Immunoreactive with sera of HCV- infected individuals.
Format
Purity:
>95.0% pure as determined by 10% PAGE (coomassie staining).
Purification Method: S-Sepharose > Ceramic Hydroxyapatite > Affinity Purification.
State:
50mM NaPO4, pH 8.5, 2.4mM EDTA, 5mM DTT, 0.1% SDS
Purified
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