AR10627PU-N HIV-2 gp36 env (His-tagged)

HIV-1 and HIV-2 appear to package their RNA differently. HIV-1 binds to any appropriate RNA whereas HIV-2 preferentially binds to mRNA which creates the Gag protein itself. This means that HIV-1 is better able to mutate. HIV-2 is transmitted in the same ways as HIV-1: Through exposure to bodily fluids such as blood, semen, tears and vaginal fluids. Immunodeficiency develops more slowly with HIV-2.
HIV-2 is less infectious in the early stages of the virus than with HIV-1.
The infectiousness of HIV-2 increases as the virus progresses.
Major differences include reduced pathogenicity of HIV-2 relative to HIV-1, enhanced immune control of HIV-2 infection and often some degree of CD4-independence. Despite considerable sequence and phenotypic differences between HIV-1 and 2 envelopes, structurally they are quite similar. Both membrane-anCHO (Chinese Hamster Ovary)red proteins eventually form the 6-helix bundles from the N-terminal and C-terminal regions of the ectodomain, which is common to many viral and cellular fusion proteins and which seems to drive fusion. HIV-1gp41 helical regions can form more stable 6-helix bundles than HIV-2gp41 helical regions however HIV-2 fusion occurs at a lower threshold temperature (25°C), does not require Ca²⁺ in the medium, is insensitive to treatment of target cells with cytochalasin B, and is not affected by target membrane glycosphingolipid composition.

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Description:

HIV-2 gp36 His tag recombinant- is a 64 kDa protein and contains the sequence of HIV-2 envelope immunodominant regions gp36 having a 6X His tag / chaperone protein on the N-terminus.
Specificity: Immunoreactive with all sera of HIV-2 infected individuals.

Buffer System:

20mM Sodium Carbonate pH 9.6

Preservatives:

0.02% Sodium Azide

Purity:

> 95.0% as determined by HPLC analysis and SDS-PAGE

State:

Liquid sterile filtered colorless clear solution
Purified