AR03020PU-S HSPA8 / HSC70 (active)

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50 µg / €275.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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HSPA8 / HSC70

AR03020PU-S

Product Description for HSPA8 / HSC70

HSPA8 / HSC70.
Presentation: Aff - Purified

Properties for HSPA8 / HSC70

Product Category Proteins & Growth Factors
Target Category
Quantity 50 µg
Synonyms HSP73, HSPA10, Heat shock 70 kDa protein 8, Heat shock cognate 71 kDa protein
Presentation Aff - Purified
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P11142
Purity > 90 % pure as determined by SDS-PAGE analysis.
Application ATPase Assay, WB control, Binding Assays, ELISA reference standard.
This protein has ATPase activity at the time of manufacture of 3.2 uM phosphate liberated/hr/ug protein in a 200 uL reaction at 37 C (pH 7.5) in the presence of 20 uL of 1 mM ATP using a Malachite Green assay.
Background Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (2). The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5).
When cells are subjected to metabolic stress (e.g., heat shock) a member of the hsp 70 family, hsp 70 (hsp72), is expressed; hsp 70 is highly related to hsc70 (>90% sequence identity). Constitutively expressed hsc70 rapidly forms a stable complex with the highly inducible hsp70 in cells following heat shock. The interaction of hsc70 with hsp 70 is regulated by ATP. These two heat shock proteins move together in the cell experiencing stress. Furthermore, research on hsc70 has implicates it with a role in facilitating the recovery of centrosomal structure and function after heat shock (6).
Concentration 2.1 mg/ml
General Readings
  1. Brown, C. L. et al. (1993) J.Cell Biol., 120 (5): 1101-1112
  2. Boorstein, W. R., Ziegelhoffer, T. & Craig, E. A. (1993), J. Mol. Evol. 38(1): 1-17.
  3. Rothman, J. (1989), Cell 59, 591 -601.
  4. DeLuca-Flaherty et al. (1990), Cell 62, 875-887.
  5. Bork, P., Sander, C. & Valencia, A. (1992). Proc. Nut1 Acad. Sci. USA 89: 7290-7294.
  6. Brown, C. L. et al. (1996) J. Biol. Chem., 271 (2): 833-840.
Storage Store the antibody (in aliquots) at -20°C.
Can be shipped at 2-8 °C.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Source E. coli
Description
Description:
Recombinant Human Hsc70 Protein with ATPase activity, his-tagged
Format
Buffer System:
Na-Phosphate, pH 7.5 (20 mM), 150 mM NaCl, 10% Glycerol, 200 mM Imidazole.
Purity:
>90% pure as determined by SDS-PAGE analysis.
State:
Liquid affinity purified protein
Aff - Purified
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