AR51994PU-S Idnk (1-187, His-tag)

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20 µg / €180.00
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Quick Overview

Idnk

Product Description for Idnk

Idnk.
Presentation: Purified

Properties for Idnk

Product Category Proteins & Growth Factors
Quantity 20 µg
Synonyms D-gluconate kinase, ECK4261, JW4225, gntV, thermosensitive
Presentation Purified
Molecular weight 23.4 kDa
Source E. coli
Species (Protein) E. coli
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P39208
Purity > 90 % by SDS - PAGE
Background D-glucote kise, thermosensitive, also known as idnk, D-glucote kise, thermosensitive, also known as idnk, is a 187 amino acid protein that belongs to the gluconokise gntK/gntV family and catalyzes the conversion of ATP and D-glucote to ADP and 6-phospho-D-glucote. This protein is thought to play a role in gender determition, deletion of the distal portion of 9p can lead to development of male to female sex reversal, the phenotype of a female with a male X, Y genotype. Recombint E.coli idnk protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventiol chromatography techniques.
Concentration 1.0 mg/ml (determined by bradford assay)
General Readings Coppo P., et al. (2006) J Haematol. 134: 171-179.Zheng X., et al. (2006) BMC cancer. 7: 262.
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGSMAGESFI LMGVSGSGKT LIGSKVAALL SAKFIDGDDL HPAKNIDKMS QGIPLSDEDR LPWLERLNDA SYSLYKKNET GFIVCSSLKK QYRDILRKGS PHVHFLWLDG DYETILARMQ RRAGHFMPVA LLKSQFEALE RPQADEQDIV RIDINHDIAN VTEQCRQAVL AIRQNRICAK EGSASDQRCE
Molecular weight:
(210aa), confirmed by MALDI-TOF
Specific activity:
Specific activity is > 80 units/mg, in which one unit will convert 1.0 umole of D-gluconate to 6-phospho-D-gluconate per minute at pH 8.0 at 37C.
Format
Buffer System:
20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT.
Purity:
>90% by SDS - PAGE
State:
Liquid purified protein
Purified
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