PBL11200-1 IFN-alpha A/D Bgl II (Universal Type I Interferon)

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1 x 10⁵ U / €350.00
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IFN-alpha A/D Bgl II (Universal Type I Interferon)

Product Description for IFN-alpha A/D Bgl II (Universal Type I Interferon)

IFN-alpha A/D Bgl II (Universal Type I Interferon).

Properties for IFN-alpha A/D Bgl II (Universal Type I Interferon)

Product Category Proteins & Growth Factors
Quantity 1 x 10⁵ U
Synonyms IFN alpha, Interferon alpha
Molecular weight 19.4 kDa
Source E. coli
Specactivity x10 U/mg
Species (Protein) Human
Shipping to Europe only
PDF datasheet View Datasheet
Manufacturer PBL Assay Science

Datasheet Extract

Purity > 95 % Purified from E. coli by a combination of hydrophobic interaction, ion exchange and gel permeation chromatography
Add. information The units are determined by use of a Human IFN Alpha A (Hu-IFN-αA [2a]) laboratory standard calibrated to the international reference standard for Human Interferon Alpha A (Hu-IFN-αA [2a]) provided by the National Institutes of Health [Meager, et al. (2001) J. Immunol. Methods. 257(1-2):17-33]. On L929 cells the laboratory standard has been calibrated to the reference standard for Mouse Interferon Alpha provided by the National Institutes of Health. Lot Activity was derived from multiple determinations in the above assay. Please note that IFN assays vary between labs and assay systems [Meager, et al (2001). J. Immunol. Meth. 257:17. Meager and Das (2005) J. Immunol. Meth. 306:1].
Source Construct described in Rehberg, E., (1982) J. Biol. Chem. 257: 11497 derived from E. coli
Description
Description:
Recombinant Human Interferon Alpha hybrid protein; Universal Type I IFN is an alpha interferon hybrid (Human Interferon Alpha A/D [BglII]) constructed from recombinant Human IFN alpha A and Human IFN alpha D. Activity has been observed on many mammalian cells, including human, monkey, mouse, bovine, rat, cat, pig, rabbit, guinea pig, or hamster. This product is useful for cross-species testing with Interferon or for species where autologous interferon is not available.
Biological activity:
Assay Used to Measure Bioactivity: Interferon was titrated in the Cytopathic Effect Inhibition (CPE) assays as listed.
Bovine (MDBK/VSV) – performed as described [Rubinstein, et al. (1981) J. Virol. 37(2):755]. The EC50 for interferon in this assay is ~5 U/ml.
Human (A549/EMCV) – performed as described [Budd, et al. (1985) Canc. Chem. Pharm. 12:39]. The EC50 for interferon in this assay is ~1 U/ml. 
Mouse (L929/EMCV) – developed and performed at PBL Biomedical Laboratories. The EC50 for interferon in this assay is ~5 U/ml.
Specific activity:
x 10 units/mg (MDBK/VSV)
Format
Buffer System:
Phosphate buffered saline (PBS) containing 0.1% bovine serum albumin (BSA)
Endotoxin level:
< 1 EU/μg
Purity:
>95% Purified from E. coli by a combination of hydrophobic interaction, ion exchange and gel permeation chromatography
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