PA1064XC IL-1 alpha (Interleukin-1 alpha)

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IL-1 alpha (Interleukin-1 alpha)

Product Description for IL-1 alpha (Interleukin-1 alpha)

IL-1 alpha (Interleukin-1 alpha).
Presentation: Purified

Properties for IL-1 alpha (Interleukin-1 alpha)

Product Category Proteins & Growth Factors
Quantity 0.1 mg
Synonyms IL1 alpha
Presentation Purified
Molecular weight 18 kDa
Source E. coli
Species (Protein) Rat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 98 % Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Add. information Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.751 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-1 as a Reference Standard.
Background Interleukin-1 alpha is a proinflammatory cytokine produced by a wide variety of cell types, including macrophages, osteoblasts, monocytes and hepatocytes. Circulating levels of are normally low and only rise after stimulation by agents such as those produced byinflammation, infection or microbial endotoxins. IL-1 alpha possesses a wide variety of biological activities and exerts its effects by binding to specific cell surface receptors.
Concentration 1 mg/ml
General Readings
  1. Mehta PA, Eapen M, Klein JP, Gandham S, Elliott J, Zamzow T, et al. Interleukin-1 alpha genotype and outcome of unrelated donor haematopoietic stem cell transplantation for chronic myeloid leukaemia. Br J Haematol. 2007 Apr;137(2):152-7. PubMed PMID: 17391495. (Free PMC Article available, 2 images available)
  2. Interleukin-1 alpha but not interleukin-1 beta gene polymorphism is associated with polycystic ovary syndrome. J Reprod Immunol 2007 Apr;73(2):188-93
  3. Birol A, Kisa U, Kurtipek GS, Kara F, Kocak M, Erkek E, et al. Increased tumor necrosis factor alpha (TNF-alpha) and interleukin 1 alpha (IL1-alpha) levels in the lesional skin of patients with nonsegmental vitiligo. Int J Dermatol. 2006 Aug;45(8):992-3. PubMed PMID: 16911396.
Storage Lyophilized Interleukin-1a although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution IL1A should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Source E. coli
Interleukin-1A Rat Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 155amino acids. The IL-1A is purified by proprietary chromatographic techniques.
AA Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-His-Ser-Phe.
Biological activity:
The ED50 as determined by the dose-dependant stimulation of murine D10S cells is < 0.005 ng/ml, corresponding to a Specific Activity of 2 x 108 IU/mg.
Molecular weight:
17703 Dalton.
Buffer System:
The protein was lyophilized from a concentrated sterile solution containing 50mM Tris-HCL, pH=8.
>98% Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Sterile Filtered White lyophilized (freeze-dried) powder.
It is recommended to reconstitute the lyophilized Interleukin 1a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
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