PA1089X IL-12 (Interleukin-12)

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IL-12 (Interleukin-12)


Product Description for IL-12 (Interleukin-12)

IL-12 (Interleukin-12).
Presentation: Purified

Properties for IL-12 (Interleukin-12)

Product Category Proteins & Growth Factors
Quantity 10 µg
Synonyms IL12
Presentation Purified
Molecular weight 75 kDa
Source Insect cells
Species (Protein) Mouse
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 95.8 % Purified by proprietary chromatographic techniques, sterile filtered purity > 95.0 % as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Background IL-12 is a heterodimeric cytokine that stimulates the production of interferon gamma from T-cells and natural killer cells, and also induces differentiation of Th1 helper cells. It is an initiator of cell-mediated immunity.
Concentration 1 mg/ml (prior to lyophil.)
General Readings
  1. Chang HD, Radbruch A. The pro- and anti-inflammatory potential of interleukin-12. Ann N Y Acad Sci. 2007 Aug;1109:40-6. PubMed PMID: 17785289.
  2. Del Vecchio M, Bajetta E, Canova S, Lotze MT, Wesa A, Parmiani G, et al. Interleukin-12: biological properties and clinical application. Clin Cancer Res. 2007 Aug 15;13(16):4677-85. PubMed PMID: 17699845.
  3. Ahuja SS, Estrada CA, Lindsey ML. Crosstalk between cytotoxic T-lymphocyte associated antigen-4 and interleukin-12 in cytotoxic T-lymphocyte-mediated myocarditis: adding another link to the chain. Circ Res. 2007 Aug 3;101(3):218-20. PubMed PMID: 17673678.
  4. Figueiredo F, Commodaro AG, de Camargo MM, Rizzo LV, Belfort R. NK1.1 cells downregulate murine endotoxin-induced uveitis following intraocular administration of interleukin-12. Scand J Immunol. 2007 Aug-Sep;66(2-3):329-34. PubMed PMID: 17635810.
  5. Zhu S, Waguespack M, Barker SA, Li S. Doxorubicin directs the accumulation of interleukin-12 induced IFN gamma into tumors for enhancing STAT1 dependent antitumor effect. Clin Cancer Res. 2007 Jul 15;13(14):4252-60. PubMed PMID: 17634555.
  6. Wulff H, Krieger T, Krüger K, Stahmer I, Thaiss F, Schäfer H, et al. Cloning and characterization of an adenoviral vector for highly efficient and doxycycline-suppressible expression of bioactive human single-chain interleukin 12 in colon cancer. BMC Biotechnol. 2007 Jun 26;7:35. PubMed PMID: 17594499. (Free PMC Article available, 7 images available)
Storage Lyophilized product is stable at room temperature for one month, should be stored desiccated below -20 °C. Upon reconstitution it should be stored at 2 - 8 °C up to one week and for future use below -20 °C. For long term storage it is recommended to add a carrier protein (0.1 % HSA or BSA). Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Source Insect cells
Interleukin-12 Mouse Recombinant produced in Sf9 insect cells is a glycosylated disulfide linked heterodimeric polypeptide containing 506 amino acids.
AA Sequence:
The sequence of the first five N-terminal amino acids was determined and found to be Arg-Val-Ile-Pro-Val at the p35 subunit and Met-Thr-Glu-Leu-Glu at the p40 subunit.
Biological activity:
The ED50 was determined by the stimulation of IFN-gamma production by murine splenocyes co-stimulated with IL-12 and was found to be < 0.1ng/ml, corresponding to a specific activity of > 1 x 10e7 IU/mg.
Molecular weight:
75 kDa comprised of disulfide-bonded 35 kDa (p35) and 40 kDa (p40) subunits.
>95.8% Purified by proprietary chromatographic techniques, sterile filtered purity > 95.0 % as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
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