AR50863PU-S Immunoglobulin J chain (23-159, His-tag)

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0.1 mg / €300.00
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Immunoglobulin J chain

Product Description for Immunoglobulin J chain

Immunoglobulin J chain.
Presentation: Purified

Properties for Immunoglobulin J chain

Product Category Proteins & Growth Factors
Target Category
Quantity 0.1 mg
Synonyms IGCJ, IGJ
Presentation Purified
Molecular weight 18.0 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P01591
Purity > 85 % by SDS - PAGE
Background Immunoglobulin J chain, also known as IGJ, serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces larger polymers. It also helps to bind these immunoglobulins to secretory component.
Concentration 0.5 mg/ml (determined by Bradford assay)
General Readings
  1. Liu T, Qian WJ, Gritsenko MA, Camp DG, Monroe ME, Moore RJ, et al. Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry. J Proteome Res. 2005 Nov-Dec;4(6):2070-80. PubMed PMID: 16335952. (Free PMC Article available, 4 images available)
  2. Ramachandran P, Boontheung P, Xie Y, Sondej M, Wong DT, Loo JA. Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry. J Proteome Res. 2006 Jun;5(6):1493-503. PubMed PMID: 16740002.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant human IGJ protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGSQEDERIV LVDNKCKCAR ITSRIIRSSE DPNEDIVERN IRIIVPLNNR ENISDPTSPL RTRFVYHLSD LCKKCDPTEV ELDNQIVTAT QSNICDEDSA TETCYTYDRN KCYTAVVPLV YGGETKMVET ALTPDACYPD
Molecular weight:
(160aa) confirmed by MALDI-TOF
Format
Buffer System:
20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Purity:
>85% by SDS - PAGE
State:
Liquid purified protein
Purified
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