AR50084PU-N Intein (3-518, His-tag)

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0.5 mg / €730.00
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Quick Overview

Intein

Product Description for Intein

Intein.
Presentation: Purified

Properties for Intein

Product Category Proteins & Growth Factors
Quantity 0.5 mg
Synonyms Intein-CBD
Presentation Purified
Molecular weight 59.4 kDa
Source E. coli
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 95 % by SDS - PAGE
Background Intein-CBD, also known as Intein, is a segment of a protein that is able to excise itself and rejoin the remaining portions with a peptide bond. Most reported inteins also contain an endonuclease domain that plays a role in intein propagation. In fact, many genes have unrelated intein-coding segments inserted at different positions. Since then, inteins have been found in all three domains of life (eukaryotes, bacteria, and archaea) and in viruses.
Concentration 1 mg/ml (determined by Bradford assay)
General Readings
  1. Goqarten JP (2006) BMC Evol Biol. 13:6-94.
  2. Anraku Y, Mizutani R, Satow Y. Protein splicing: its discovery and structural insight into novel chemical mechanisms. IUBMB Life. 2005 Aug;57(8):563-74. PubMed PMID: 16118114.
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant Bacillus circulans Intein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. 
N-terminal Sequence Analysist: Met-Gly-Pro-Thr-Gly
AA Sequence:
MKIEEGKLVI GSLEGCFAKG TNVLMADGSI ECIENIEVGN KVMGKDGRPR EVIKLPRGRE TMYSVVQKSQ HRAHKSDSSR EVPELLKFTC NATHELVVRT PRSVRRLSRT IKGVEYFEVI TFEMGQKKAP DGRIVELVKE VSKSYPISEG PERANELVES YRKASNKAYF EWTIEARDLS LLGSHVRKAT YQTYAPILYE NDHFFDYMQK SKFHLTIEGP KVLAYLLGLW IGDGLSDRAT FSVDSRDTSL MERVTEYAEK LNLCAEYKDR KEPQVAKTVN LYSKVVRGAS TNPGVSAWQV NTAYTAGQLV TYNGKTYKCL QPHTSLAGWE PSNVPALWQL QGGHGGIRNN LNTENPLWDA IVGLGFLKDG VKNIPSFLST DNIGTRETFL AGLIDSDGYV TDEHGIKATI KTIHTSVRDG LVSLARSLGL VVSVNAEPAK VDMNVTKHKI SYAIYMSGGD VLLNVLSKCA GSKKFRPAPA AAFARECRGF YFELQELKED DYYGITLSDD SDHQFLLGSQ VVVQNLEHHH HHH
Molecular weight:
(533aa), confirmed by MALDI-TOF
Format
Buffer System:
20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
Purity:
>95% by SDS - PAGE
State:
Liquid purified protein
Purified
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