discontinued

PA1040 Interferon-alpha A (IFN-a2a) (mPEG-ALD)

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2 µg / €110.00

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Interferon-alpha A (IFN-a2a) (mPEG-ALD)

PA1040

Product Description for Interferon-alpha A (IFN-a2a) (mPEG-ALD)

Interferon-alpha A (IFN-a2a) (mPEG-ALD).
Presentation: Purified

Properties for Interferon-alpha A (IFN-a2a) (mPEG-ALD)

Product Category Proteins & Growth Factors
Quantity 2 µg
Synonyms IFN alpha, Interferone alpha
Presentation Purified
Molecular weight 59 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 98 % Greater than 98% as determined by HPLC and SDS PAGE.
Background Adiponectin (also called ACRP30 and GBP28) is a protein exclusively secreted from adipose tissue. In the circulation, adiponectin is present as three different oligomeric complexes, including the high molecular weight (HMW), the middle molecular weight (MMW, also called hexamer) and low molecular weigh (LMW, also called trimer) forms. Different oligomeric complex of adiponectin activates different signaling pathways and exerts distinct functions.
Concentration 0.6 mg/ml
General Readings
  1. Waki H, Yamauchi T, Kamon J, Kita S, Ito Y, Hada Y, et al. Generation of globular fragment of adiponectin by leukocyte elastase secreted by monocytic cell line THP-1. Endocrinology. 2005 Feb;146(2):790-6. Epub 2004 Nov 4. PubMed PMID: 15528304.
  2. Xu A, Wang Y, Keshaw H, Xu LY, Lam KS, Cooper GJ. The fat-derived hormone adiponectin alleviates alcoholic and nonalcoholic fatty liver diseases in mice. J Clin Invest. 2003 Jul;112(1):91-100. PubMed PMID: 12840063. (Free PMC Article available, 10 images available)
  3. Pajvani UB, Du X, Combs TP, Berg AH, Rajala MW, Schulthess T, et al. Structure-function studies of the adipocyte-secreted hormone Acrp30/adiponectin. Implications fpr metabolic regulation and bioactivity. J Biol Chem. 2003 Mar 14;278(11):9073-85. Epub 2002 Dec 20. PubMed PMID: 12496257.
  4. Wang Y, Xu A, Knight C, Xu LY, Cooper GJ. Hydroxylation and glycosylation of the four conserved lysine residues in the collagenous domain of adiponectin. Potential role in the modulation of its insulin-sensitizing activity. J Biol Chem. 2002 May 31;277(22):19521-9. Epub 2002 Mar 23. PubMed PMID: 11912203.
  5. Yamauchi T, Kamon J, Minokoshi Y, Ito Y, Waki H, Uchida S, et al. Adiponectin stimulates glucose utilization and fatty-acid oxidation by activating AMP-activated protein kinase. Nat Med. 2002 Nov;8(11):1288-95. Epub 2002 Oct 7. PubMed PMID: 12368907.
Storage For long term, store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized protein remains stable for 24 months when stored at -20°C.
Source E.coli.
Description
Description:
Total 170AA. N-terminal underlined amino acids are His-tag and the protease cleavage site (31AA). The AA sequence is homologous to the 105-244 amino acid sequence of the Human full-length Adiponectin.
AA Sequence:
MSWWHHHHHH NWNIPTTQDT TQDLWFEGAM GGEGAYVYRS FSVGLETYV TIPNMPIRFT KIFYNQQNHYDGSTGKFHCN IPGLYYFAYH ITVYMKDVKV SLFKKDKAML FTYDQYQENN VDQASGSVLL HEVGDQVWLQVYGEGERNGL YADNDNDSTF TGFLLYHDTN (Swiss-prot entry Q15848)

Applications: ELISA, Western blotting, cell culture and/or animal studies
Other applications not tested. Optimal dilutions are dependent of conditions and should be determined by the user.
Format
Buffer System:
Sterile filtered and lyophilized from PBS buffer.
Purity:
>98% Greater than 98% as determined by HPLC and SDS PAGE.
State:
Sterile Filtered White lyophilized (freeze-dried) powder.
Purified
Reconstitution:
Add 0.2 ml of deionized water and let the lyophilized pellet dissolve completely.
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