discontinued

AR10465PU-N Interferon tau

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10 µg / €300.00

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Interferon tau

AR10465PU-N

Product Description for Interferon tau

Interferon tau.
Presentation: Purified

Properties for Interferon tau

Product Category Proteins & Growth Factors
Quantity 10 µg
Presentation Purified
Molecular weight 19914.7 Da
Source E. coli
Species (Protein) Sheep
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P56828
Purity >95.0% as determined by both RP-HPLC and SDS-PAGE analysis. 
Purification Method: Proprietary chromatographic techniques.
Background

IFN-tau is also known as TP-1 (trophoblast protein-1) is a new class of type I IFN that is secreted by the trophoblast and is the signal for maternal recognition of pregnancy in sheep. IFN- tau has potent immunosuppressive and antiviral activities similar to other type I IFN but is less cytotoxic than IFN-alpha and IFN-beta. The current investigation concerns the effect of recombinant ovine IFN- tau (rOIFN- tau) on the modulation of MHC class I and II expression on cloned mouse cerebrovascular endothelial (CVE) cells.
IFN-tau induced tyrosine phosphorylation of Stat1 and upregulated the expression of MHC class I on CVE. One proposed action by which type I IFN reduces the relapse rate in MS is via interference with IFN-gamma-induced MHC class II expression. IFN-tau was shown to downregulate IFN-gamma-induced MHC class II expression on CVE and, hence, may be of potential therapeutic value in downregulating inflammation in the central nervous system (CNS). IFN- tau did not upregulate the expression of MHC class II on CVE. IFN-tau also inhibited the replication of Theiler's virus in CVE.

Concentration 1.0 mg/ml (prior to lyophil.)
Storage Prior to reconstitution store at 2-8°C for one month or desiccated below -18°C.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer. 
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). 
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant Ovine Interferon-Tau produced in E.coli is a single, non-glycosylated, polypeptide chain containing 172 amino acids.
Protein content: Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.027 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IFN-Tau as a Reference Standard.
AA Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Tyr-Leu-Ser-Arg.
Biological activity:
The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 1 x 107 IU/mg.
Format
Buffer System:
Lyophilized from (1mg/ml) solution containing PBS, pH 7.4
Purity:
>95.0% as determined by both RP-HPLC and SDS-PAGE analysis. 
Purification Method: Proprietary chromatographic techniques.
State:
Sterile filtered white lyophilized (freeze-dried) powder
Purified
Reconstitution:
Restore in sterile 18MΩ-cm-1 H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
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