discontinued

PA1065 Interleukin-1 beta / IL-1B

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Interleukin-1 beta / IL-1B

Product Description for Interleukin-1 beta / IL-1B

Interleukin-1 beta / IL-1B.
Presentation: Purified

Properties for Interleukin-1 beta / IL-1B

Product Category Proteins & Growth Factors
Target Category
Quantity 2 µg
Synonyms Catabolin, IL-1 beta, IL1 beta, IL1B, IL1F2
Presentation Purified
Molecular weight 17 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P01584
Purity > 98 % pure as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Purified by proprietary chromatographic techniques
Add. information Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.631 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of Recombinant IL-1 beta as a reference standard.
Background Interleukin-1b is produced by activated macrophages; IL-1b stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1b proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
General Readings
  1. Gershanovich ML, Filatova LV, Ketlinsky SA, Simbirtsev AS. Recombinant human interleukin-1 beta: new possibilities for the prophylaxis and correction of toxic myelodepression in patients with malignant tumors. II. Phase II study of the protective effect of recombinant human interleukin-1 beta on myelodepression induced by chemotherapy in cancer patients. Eur Cytokine Netw. 2001 Oct-Dec;12(4):671-5. PubMed PMID: 11781195.
  2. Gershanovich ML, Filatova LV, Ketlinsky SA, Simbirtsev AS. Recombinant human interleukin-1 beta: new possibilities for the prophylaxis and correction of toxic myelodepression in patients with malignant tumors. I. Phase I-II clinical trials of recombinant human interleukin-1 beta as a leukopoiesis stimulator in cancer patients receiving combination chemotherapy. Eur Cytokine Netw. 2001 Oct-Dec;12(4):664-70. PubMed PMID: 11781194.
  3. Chen NN, Wu SG. [Effect of recombinant human interleukin-1 beta on K+ channels of mice bone marrow stromal cells]. Sheng Li Xue Bao. 1999 Dec;51(6):637-44. PubMed PMID: 11498933.
  4. Abramov VM, Vasiliev AM, Vasilenko RN, Kulikova NL, Kosarev IV, Khlebnikov VS, et al. Structural and functional similarity between Yersinia pestis capsular protein Caf1 and human interleukin-1 beta. Biochemistry. 2001 May 22;40(20):6076-84. PubMed PMID: 11352744.
  5. Iwasaki LR, Haack JE, Nickel JC, Reinhardt RA, Petro TM. Human interleukin-1 beta and interleukin-1 receptor antagonist secretion and velocity of tooth movement. Arch Oral Biol. 2001 Feb;46(2):185-9. PubMed PMID: 11163326.
Storage Lyophilized IL-1 beta although stable at room temperature for 3 weeks, should be stored desiccated below -20°C.
Upon reconstitution IL-1 beta should be stored at 2-8°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant Human IL-1 beta produced in E.coli is a non-glycosylated polypeptide chain containing 153 amino acids having a molecular mass of 17000 Dalton.rHuIL-1b is purified by proprietary chromatographic techniques.
AA Sequence:
The sequence of the first five N-terminal amino acids was found to be:
Ala-Pro-Val-Arg-Ser.
Biological activity:
Interleukin-1 beta is fully biologically active when compared to standard.
Specific activity:
The specific activity as determined in the test of augmentation of lymphocyte proliferation assay using mouse thymus was found to be 2.0 x 10e8 IU/mg.
Format
Buffer System:
The protein was lyophilized from a concentrated (1 mg/ml) sterile solution containing 50 mM Phosphate buffer, pH 7.1, and 150 mM NaCl.
Purity:
>98% pure as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Purified by proprietary chromatographic techniques
State:
Sterile filtered white freeze-dried powder
Purified
Reconstitution:
It is recommended to reconstitute the lyophilized IL-1 beta in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
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