AR09050PU-L Interleukin-15 / IL15 (49-162, His-tag)

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0.5 mg / €1,000.00
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Interleukin-15 / IL15

AR09050PU-L

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  • AR09050PU-L

Product Description for Interleukin-15 / IL15

Interleukin-15 / IL15.
Presentation: Purified

Properties for Interleukin-15 / IL15

Product Category Proteins & Growth Factors
Target Category
Quantity 0.5 mg
Synonyms IL-15
Presentation Purified
Molecular weight 13.9 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P40933
Purity > 95 % by SDS PAGE
Background Interleukin (IL-15) is a pleiotropic cytokine that plays a pivotal role in both innate and adaptive immunity. IL-15 is unique among cytokines due to its participation in a trans signaling mechanism in which IL-15 receptor alpha (IL-15R alpha) from one subset of cells presents IL-15 to neighboring IL-2R beta/gamma(c)-expressing cells. This newly discovered cytokine is produced by a wide variety of cells and tissues and like IL-2, stimulates the proliferation of T-lymphocytes and induces the generation of cytotoxic T-lymphocytes (CTLs) and lymphokine-activated killer (LAK) cells.
Concentration 1.0 mg/ml
General Readings Lorenzen I., et al., (2006). J Biol Chem. Mar 10; 281 (10):6642-7.
Storage Store (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant human IL-15, fused to His-tag, was expressed in E.coli and purified by using conventional chromatography after refolding of the isolated inclusion bodies in a redox buffer.
AA Sequence:
MNWVNVISDL KKIEDLIQSM HIDATLYTES DVHPSCKVTA MKCFLLELQV ISLESGDASI HDTVENLIIL ANNSLSSNGN VTESGCKECE ELEEKNIKEF LQSFVHIVQM FINTSLEHHH HHH
Biological activity:
Measured in a cell proliferation assay using CTLL2 mouse cytotoxic
T cells. The ED50 for this effect is < 2.5 ng/ml.
Activity Assay
1.Cell line: CTLL2 (mouse cytotoxic T cell)
2.Maintenance Condition: 10% FBS RPMI 1640 with hIL2
3.Assay Medium: 10% FBS RPMI 1640 without hIL2
4.Cell Density: 2 x 10e4 cells/well (96 well plate, final volume 100ul)
5.Starvation : 24hr, Assay medium
6.Incubation Time: 24hr (after sample treatment)
7.Concentration Range: 0.039 ng/ml - 40 ng/ml
8.Detection Method : MTT assay
Molecular weight:
(123 aa), confirmed by MALDI-TOF
Format
Buffer System:
PBS, pH 7.4, 0.2 mM PMSF, 10% glycerol
Endotoxin level:
< 1.0 EU per 1 μg of protein (determined by LAL method)
Purity:
>95% by SDS PAGE
State:
Liquid purified protein
Purified
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