PA1079XC Interleukin-6 (IL-6)

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1 mg / €2,130.00

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Interleukin-6 (IL-6)


Product Description for Interleukin-6 (IL-6)

Interleukin-6 (IL-6).
Presentation: Purified

Properties for Interleukin-6 (IL-6)

Product Category Proteins & Growth Factors
Quantity 1 mg
Synonyms BSF-2, BSF2, CDF, HGF, HSF, IFNB2, IL6, Interferon beta-2, Interleukin 6, MGI-2A
Presentation Purified
Molecular weight 21 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 98 % Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Add. information Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.47 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-6 as a reference standard.
Background Cytokines of the IL6/GCSF/MGF family are glycoproteins of about 170 to 180 amino acid residues that contains four conserved cysteine residues involved in two disulphide bonds.They have a compact, globular fold (similar to other interleukins), stabilised by the 2 disulphide bonds. One half of the structure is dominated by a 4 alpha-helix bundle with a left-handed twist: the helices are anti-parallel, with 2 overhand connections, which fall into a 2-stranded anti-parallel beta-sheet. The fourth alpha-helix is important to the biological activity of the molecule .
Interleukin (IL)-6 is an important proinflammatory and immunoregulatory cytokine expressed by various cells. Interleukin-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro.
General Readings 1. Production of soluble human interleukin-6 in cytoplasm by fed-batch culture of recombinant E. coli. Biotechnol Prog 2005 Mar-Apr;21(2):524-31
2. Recombinant human interleukin-6 infusion during low-intensity exercise does not enhance whole body lipolysis or fat oxidation in humans.
Am J Physiol Endocrinol Metab 2005 Jul;289(1):E2-7
3. Kaposi's sarcoma-associated herpesvirus-encoded viral interleukin-6 is secreted and modified differently than human interleukin-6: evidence for a unique autocrine signaling mechanism. J Biol Chem 2004 Dec 10;279(50):51793-803
4. Two novel somatic mutations in the human interleukin 6 promoter region in a patient with sporadic breast cancer. Eur J Immunogenet 2003 Dec;30(6):397-400
5. Cellular cholesterol depletion triggers shedding of the human interleukin-6 receptor by ADAM10 and ADAM17 (TACE). J Biol Chem 2003 Oct 3;278(40):38829-39
6. Effect of in situ expression of human interleukin-6 on antibody responses against Salmonella typhimurium antigens. FEMS Immunol Med Microbiol 2003 Jul 15;37(2-3):135-45
Storage Lyophilized rHuIL-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C.
Upon reconstitution rHuIL-6 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Shelf life: one year from despatch.
Source E. coli
Recombinant Human IL-6 produced in E.coli is a single, non-glycosylated polypeptide chain containing 184 amino acids and having a molecular mass of 21000 Dalton.The sequence of the first five N-terminal amino acids was determined and was found to be Met-Pro-Val-Pro-Pro. rHuIL-6 is purified by proprietary chromatographic techniques.
Biological activity:
The ED50 as determined by dose-dependent stimulation of murine 7TD1 cells is < 0.1 ng/ml.
Buffer System:
Lyophilized from a 1.0 mg/ml solution in PBS, pH 7.4
>98% Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
0.2 µm sterile filtered, freeze-dried
It is recommended to reconstitute the lyophilized rHuIL-6 in sterile
18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
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