PA1080XC Interleukin-6 (IL-6)

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Interleukin-6 (IL-6)

Product Description for Interleukin-6 (IL-6)

Interleukin-6 (IL-6).
Presentation: Purified

Properties for Interleukin-6 (IL-6)

Product Category Proteins & Growth Factors
Quantity 1 mg
Synonyms BSF-2, BSF2, CDF, HGF, HSF, IFNB2, IL6, Interferon beta-2, Interleukin 6, MGI-2A
Presentation Purified
Molecular weight 22 kDa
Source E. coli
Species (Protein) Mouse
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 98 % Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Background Interleukin-6 is a potent pro-inflammatory cytokine primarily produced by activated T cells and an assortment of other cells including endothelial cells and macrophages. IL-6 affects B and T lymphocytes and has been shown to have a role in host defense, acute phase reactions, immune responses and hematopoiesis.
Concentration 1 mg/ml
General Readings
  1. Kim TW, Chung BH, Chang YK. Production of soluble human interleukin-6 in cytoplasm by fed-batch culture of recombinant E. coli. Biotechnol Prog. 2005 Mar-Apr;21(2):524-31. PubMed PMID: 15801793.
  2. Hiscock N, Fischer CP, Sacchetti M, van Hall G, Febbraio MA, Pedersen BK. Recombinant human interleukin-6 infusion during low-intensity exercise does not enhance whole body lipolysis or fat oxidation in humans. Am J Physiol Endocrinol Metab. 2005 Jul;289(1):E2-7. Epub 2005 Mar 1. PubMed PMID: 15741245.
  3. Meads MB, Medveczky PG. Kaposi's sarcoma-associated herpesvirus-encoded viral interleukin-6 is secreted and modified differently than human interleukin-6: evidence for a unique autocrine signaling mechanism. J Biol Chem. 2004 Dec 10;279(50):51793-803. Epub 2004 Jul 16. PubMed PMID: 15258150.
  4. Saha A, Bairwa NK, Ranjan A, Gupta V, Bamezai R. Two novel somatic mutations in the human interleukin 6 promoter region in a patient with sporadic breast cancer. Eur J Immunogenet. 2003 Dec;30(6):397-400. PubMed PMID: 14675392.
  5. Matthews V, Schuster B, Schütze S, Bussmeyer I, Ludwig A, Hundhausen C, et al. Cellular cholesterol depletion triggers shedding of the human interleukin-6 receptor by ADAM10 and ADAM17 (TACE). J Biol Chem. 2003 Oct 3;278(40):38829-39. Epub 2003 Jun 27. PubMed PMID: 12832423.
  6. Li Y, Reichenstein K, Ullrich R, Danner T, von Specht BU, Hahn HP. Effect of in situ expression of human interleukin-6 on antibody responses against Salmonella typhimurium antigens. FEMS Immunol Med Microbiol. 2003 Jul 15;37(2-3):135-45. PubMed PMID: 12832117.
Storage Lyophilized Interleukin-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-6 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Source E. coli
Interleukin-6 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 187 amino acids. The IL-6 is purified by proprietary chromatographic techniques.
AA Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Pro-Thr-Ser.
Biological activity:
The ED50 as determined by the dose-dependant stimulation of murine 7TD1 cells is less then 0.02 ng/ml, corresponding to a Specific Activity of 1 x 10 7IU/mg.
Molecular weight:
21709 Dalton.
Buffer System:
The protein was lyophilized from a concentrated solution containing 50mM NH4HCO3, pH 8.0.
>98% Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Sterile Filtered White lyophilized (freeze-dried) powder.
It is recommended to reconstitute the lyophilized Interleukin 6 in 100 mM acetic acid to 0.1-1.0 mg/mL to regain full activity, and can then be further diluted to other aqueous solutions.
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