PA1081XC Interleukin-6 (IL-6)

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1 mg / €3,650.00

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Interleukin-6 (IL-6)

Product Description for Interleukin-6 (IL-6)

Interleukin-6 (IL-6).
Presentation: Purified

Properties for Interleukin-6 (IL-6)

Product Category Proteins & Growth Factors
Quantity 1 mg
Synonyms BSF-2, BSF2, CDF, HGF, HSF, IFNB2, IL6, Interferon beta-2, Interleukin 6, MGI-2A
Presentation Purified
Molecular weight 22 kDa
Source E. coli
Species (Protein) Rat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 95 % Purified by proprietary chromatographic techniques, sterile filtered, purity > 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Add. information Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.55 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-6 as a Reference Standard.
Background Interleukin-6 is a potent pro-inflammatory cytokine primarily produced by activated T cells and an assortment of other cells including endothelial cells and macrophages. IL-6 affects B and T lymphocytes and has been shown to have a role in host defense, acute phase reactions, immune responses and hematopoiesis.
Concentration 1 mg/ml (prior to lyophil.)
General Readings
  1. Saijo Y, Yoshioka E, Fukui T, Kawaharada M, Sata F, Sato H, et al. Effects of the Interaction between Interleukin-6 -634C/G Polymorphism and Smoking on Serum C-Reactive Protein Concentrations. Hypertens Res. 2007 Jul;30(7):593-9. PubMed PMID: 17785926.
  2. Are promoter polymorphisms of interleukin 6 ready to be applied in genetic markers of cardiovascular diseases? Hypertens Res 2007 Jul;30(7):575-6
  3. Gealy C, Humphreys C, Dickinson V, Stinski M, Caswell R. An activation-defective mutant of the human cytomegalovirus IE2p86 protein inhibits NF-kappaB-mediated stimulation of the human interleukin-6 promoter. J Gen Virol. 2007 Sep;88(Pt 9):2435-40. PubMed PMID: 17698652.
  4. Kobayashi K, Yokote T, Akioka T, Hara S, Oka S, Hiraoka N, et al. [Vascular endothelial growth factor and interleukin 6 production by Hodgkin lymphoma]. Gan To Kagaku Ryoho. 2007 Aug;34(8):1327-30. PubMed PMID: 17687225.
  5. Dubiński A, Zdrojewicz Z. [The role of interleukin-6 in development and progression of atherosclerosis]. Pol Merkur Lekarski. 2007 Apr;22(130):291-4. PubMed PMID: 17684929.
  6. Mukamal KJ, Jenny NS, Tracy RP, Siscovick DS. Alcohol consumption, interleukin-6 and apolipoprotein E genotypes, and concentrations of interleukin-6 and serum amyloid P in older adults. Am J Clin Nutr. 2007 Aug;86(2):444-50. PubMed PMID: 17684217. (Free PMC Article available)
Storage Lyophilized product is stable at room temperature for one month, should be stored desiccated below -20 °C. Upon reconstitution it should be stored at 2 - 8 °C up to one week and for future use below -20 °C. For long term storage it is recommended to add a carrier protein (0.1 % HSA or BSA). Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Source E. coli
Interleukin-6 Rat Recombinant is a single, non-glycosylated polypeptide chain containing 187 amino acids.
AA Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Pro-Thr-Ser.
Biological activity:
The ED50= 0.03-0.1 ng/mL. The biological activity is determined by measuring the dose-dependant proliferation of IL-6 dapendent B9 cells. A concentration range of 0.1 to 10.0 ng/mL is effective for most in vitro applications.
Molecular weight:
21732 Dalton.
>95% Purified by proprietary chromatographic techniques, sterile filtered, purity > 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
100 mM acetic acid to 1.0 mg/mL, incubated for 30 minutes at room temperature to regain full activity, which can then be further diluted to other aqueous solutions.
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