AR09233PU-L Interleukin-6 / IL6 (30-212, His-tag)

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0.5 mg / €1,000.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Interleukin-6 / IL6

Product Description for Interleukin-6 / IL6

Interleukin-6 / IL6.
Presentation: Purified

Properties for Interleukin-6 / IL6

Product Category Proteins & Growth Factors
Target Category
Quantity 0.5 mg
Synonyms B-cell stimulatory factor 2, BSF-2, CDF, CTL differentiation factor, Hybridoma growth factor, IFNB2, IL-6, Interferon beta-2
Presentation Purified
Molecular weight 23.1 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P05231
Purity > 95 % pure by SDS-PAGE
Background Interleukin-6, as known as IL-6, is an immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is also a "myokine," a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 acts as both a pro-inflammatory and anti-inflammatory cytokine. Smooth muscle cells in the tunica media of many blood vessels also produce IL-6. It is mediated through its inhibitory effects on TNF-alpha and IL-1, and activation of IL-1ra and IL-10.
Concentration 0.5 mg/ml (determined by Bradford assay)
General Readings
  • Ishihara, et al. (2002) Cytokine Growth Factor Rev. 13(4-5):357-68.
  • Vgontzas AN, et al. (2005) Neuroimmunomodulation.12, 131-40.
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant interleukin-6, fused to His-tag, was expressed in E.coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM
Biological activity:
Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 for this effects is less or equal to 0.8 ng/ml.
Molecular weight:
(204 aa)
Format
Buffer System:
PBS, pH 7.4, containing 10% Glycerol
Endotoxin level:
< 1.0 EU per 1 µg of protein (determined by LAL method)
Purity:
>95% pure by SDS-PAGE
State:
Liquid purified protein
Purified
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