discontinued

PA079 Interleukin-6 / IL6

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Interleukin-6 / IL6

PA079

Product Description for Interleukin-6 / IL6

Interleukin-6 / IL6.

Properties for Interleukin-6 / IL6

Product Category Proteins & Growth Factors
Target Category
Quantity 5 µg
Synonyms B-cell stimulatory factor 2, BSF-2, CDF, CTL differentiation factor, Hybridoma growth factor, IFNB2, IL-6, Interferon beta-2
Molecular weight 20.9 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P05231
Purity > 98 % pure by SDS-PAGE and HPLC analyses.
Add. information Centrifuge the vial prior to opening.
Background IL-6 is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 signals through the IL-6 receptor system that consists of two chains, IL-6R alpha and gp130. Murine IL-6 is inactive on human cells, while both human and murine are equally active on murine cells.
Storage The lyophilized protein is stable at -20°C for one year from date of despatch.
Reconstituted IL-6 is stable for 3 months when stored in working aliquots with a carrier protein at -20°C.
Avoid repeated freezing and thawing.
Description
Description:
Recombinant Human IL-6 is a 20.9 kDa protein containing 184 amino acid residues.
AA Sequence:
PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM
Biological activity:
The ED50 as determined by the dose-dependent stimulation of the proliferation of IL-6 dependent murine 7TD1 cells is ≤ 0.1 ng/ml.
Molecular weight:
(184 aa)
Specific activity:
≥ 1 x 107 units/mg
Format
Buffer System:
10mM Sodium Phosphate, pH 7.5
Endotoxin level:
< 0.1 ng per µg (1EU/µg)
Purity:
>98% pure by SDS-PAGE and HPLC analyses.
State:
Lyophilized (sterile filtered) purified fraction.
Reconstitution:
Restore in Water to a concentration of 0.1-0.5 mg/ml.
Note: Gently shake the solution for about 10 minutes for better solubility.
This solution can then be diluted into other aqueous buffers and stored at 2-8°C for 1 week or at -20°C for future use.
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