PA1082 Interleukin-7 (IL-7)

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Interleukin-7 (IL-7)

Product Description for Interleukin-7 (IL-7)

Interleukin-7 (IL-7).
Presentation: Purified

Properties for Interleukin-7 (IL-7)

Product Category Proteins & Growth Factors
Quantity 2 µg
Synonyms IL7
Presentation Purified
Molecular weight 17 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 98 % Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Add. information Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.418 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-7 as a Reference Standard.
Background IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival.
Concentration 1 mg/ml
General Readings 1. A three-base-deletion polymorphism in the upstream non-coding region of human interleukin 7 (IL-7) gene could enhance levels of IL-7 expression. Int J Immunogenet 2007 Apr;34(2):107-13
2. Effects of human interleukin 7 on HIV-1 replication in monocyte-derived human macrophages. AIDS 2006 Apr 4;20(6):937-9
3. Disulfide bond assignment in human interleukin-7 by matrix-assisted laser desorption/ionization mass spectroscopy and site-directed cysteine to serine mutational analysis. J Biol Chem 1997 Dec 26;272(52):32995-3000
4. Homology modeling study of the human interleukin-7 receptor complex. Protein Eng 1996 Dec;9(12):1135-42
5. Quantitative and cell-cycle differences in progenitor cells mobilized by recombinant human interleukin-7 and recombinant human granulocyte colony-stimulating factor.
Blood 1996 Dec 1;88(11):4139-48
6. Prediction of the three-dimensional structure of human interleukin-7 by homology modeling. Protein Eng 1996 Jun;9(6):493-8
Storage Lyophilized Interleukin-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL7 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Source E. coli
Interleukin-7 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 153 amino acids. The IL-7 is purified by proprietary chromatographic techniques.
AA Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Asp-Cys-Asp-Ile.
Biological activity:
The ED50 as determined by the dose-dependant stimulation of murine IXN/2B cells is less then 0.5ng/ml, corresponding to a Specific Activity of 2,000,000 IU/mg.
Molecular weight:
17412 Dalton.
Buffer System:
Lyophilized from a concentrated solution in water containing no additives.
>98% Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Sterile Filtered White lyophilized (freeze-dried) powder.
It is recommended to reconstitute the lyophilized Interleukin -7 in sterile 18MĎ?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
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