PA1083 Interleukin-7 (IL-7)

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Interleukin-7 (IL-7)


Product Description for Interleukin-7 (IL-7)

Interleukin-7 (IL-7).
Presentation: Purified

Properties for Interleukin-7 (IL-7)

Product Category Proteins & Growth Factors
Quantity 2 µg
Synonyms IL7
Presentation Purified
Molecular weight 15 kDa
Source E. coli
Species (Protein) Mouse
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 98 % Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel
Recombinant Mouse IL-7 is purified by proprietary chromatographic techniques.
Add. information Gene Name Il7
Protein synonyms/aliases: Interleukin-7 precursor (IL-7)
Protein Family: belongs to the IL-7/IL-9 family. Domains: IPR000226 Interleukin-7/interleukin-9
Background Interleukin 7 (IL-7), which is constitutively produced particularly by stromal cells from the bone marrow and thymus, plays a crucial role in T cell homeostasis. This cytokine is implicated in thymopoiesis since it sustains thymocyte proliferation and survival. It regulates peripheral naive T cell survival by modulating the expression of the anti-apoptotic molecule Bcl-2, and sustains peripheral T cell expansion in response to antigenic stimulation. Infection by the human immunodeficiency virus (HIV) leads to severe T lymphopenia and general immune dysfunction. Increased IL-7 plasma levels are generally observed in HIV-infected patients.
General Readings
  1. Roessler S, Györy I, Imhof S, Spivakov M, Williams RR, Busslinger M, et al. Distinct promoters mediate the regulation of Ebf1 gene expression by interleukin-7 and Pax5. Mol Cell Biol. 2007 Jan;27(2):579-94. Epub 2006 Nov 13. PubMed PMID: 17101802. (Free PMC Article available, 8 images available)
  2. Feyen O, Göbel U, Schneider DT, Burdach SE, Niehues T. Long-term haematopoietic reconstitution and survival without interleukin-7 in a murine syngeneic bone marrow transplantation model. Scand J Immunol. 2006 Dec;64(6):601-8. PubMed PMID: 17083616.
  3. Wallace DL, Bérard M, Soares MV, Oldham J, Cook JE, Akbar AN, et al. Prolonged exposure of naïve CD8+ T cells to interleukin-7 or interleukin-15 stimulates proliferation without differentiation or loss of telomere length. Immunology. 2006 Oct;119(2):243-53. PubMed PMID: 17005004. (Free PMC Article available, 7 images available)
  4. Faller EM, McVey MJ, Kakal JA, MacPherson PA. Interleukin-7 receptor expression on CD8 T-cells is downregulated by the HIV Tat protein. J Acquir Immune Defic Syndr. 2006 Nov 1;43(3):257-69. PubMed PMID: 16967044.
  5. Fukatsu K, Moriya T, Ikezawa F, Maeshima Y, Omata J, Yaguchi Y, et al. Interleukin-7 dose-dependently restores parenteral nutrition-induced gut-associated lymphoid tissue cell loss but does not improve intestinal immunoglobulin a levels. JPEN J Parenter Enteral Nutr. 2006 Sep-Oct;30(5):388-93; discussion 393-4. PubMed PMID: 16931606.
  6. Storek J, Nash RA, McSweeney PA, Furst DE, Sullivan KM. Normal interleukin-7 (IL7) levels and normal IL7 response to CD4 T lymphopenia in patients with multiple sclerosis and systemic sclerosis. Clin Immunol. 2006 Oct;121(1):118-9. Epub 2006 Jul 17. PubMed PMID: 16844418.
Storage Lyophilized Recombinant Murine IL-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Recombinant Murine IL-7 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. Shelf life: one year from despatch.
Source E. coli
Recombinant Murine IL-7 produced in E. coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 14897 Dalton.
AA Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Cys-His-Ile.
Biological activity:
Murine IL-7 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of murine 2E8 cells is < 0.2 ng/ml, corresponding to a Specific Activity of 2 x 106 IU/mg.
Less than 1% as determined by silver-stained SDS-PAGE gel analysis
Endotoxin level:
Less than 0.1 ng/µg (IEU/µg) of Murine IL-7
>98% Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel
Recombinant Mouse IL-7 is purified by proprietary chromatographic techniques.
Lyophilized from a concentrated (1mg/ml) solution in water containing no additives
It is recommended to reconstitute the lyophilized Murine IL-7 in sterile 18MĎ?-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
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