TP710043 Isocitrate dehydrogenase / IDH (C-term DDK tag)

Recombinant protein of mutant(R132H) of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1),with C-terminal DDK tag,expressed in sf9 cells.

Search for all "Isocitrate dehydrogenase / IDH"

20 µg / €411.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Isocitrate dehydrogenase / IDH

TP710043

More Views

  • TP710043

Product Description for Isocitrate dehydrogenase / IDH

Isocitrate dehydrogenase / IDH.

Properties for Isocitrate dehydrogenase / IDH

Product Category Proteins & Growth Factors
Target Category
Quantity 20 µg
Synonyms Cytosolic NADP-isocitrate dehydrogenase, ICDH, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, PICD, cytoplasmic Isocitrate dehydrogenase [NADP]
Molecular weight 47 kDa
Source SF9 cells
Species (Protein) Human
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
O75874
Purity > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
Add. information Tag: C-terminal DDK
Storage Stable for at least 3 months from receipt of products under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Description
Specific activity:
Enzymatic activities were determined by monitoring DPH formation based on the absorbance at 345nm. The reaction was carried out at 37? for 10 minutes in the presence of isocitrate as a substrate and DP as a cofactor. The data which presented a good linear relation on the curve was used to calculate the specific activity, and one unit is defined as converting 1.0 umole of DP to DPH per min at 37?. In summary, the wildtype IDH1 produced from HEK293 cells and insect cells are active while the R132H mutant or the WT/R132H heterodimers are ictive.
Format
Buffer System:
50mM Tris-HCl pH8.0, 150mM NaCl, 10%glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
Purity:
>80%
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
  • LinkedIn