AR31175PU-N Klotho

Search for all "Klotho"

20 µg / €330.00
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Quick Overview

Klotho

AR31175PU-N

Product Description for Klotho

Klotho.
Presentation: Purified

Properties for Klotho

Product Category Proteins & Growth Factors
Target Category
Quantity 20 µg
Synonyms EC 3.2.1.31, KL, Klotho
Presentation Purified
Molecular weight 65-70 kDa
Source CHO cells
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9UEF7
Purity > 98 % by SDS-PAGE and HPLC
Background Klotho is a glycosylated protein that plays an important role in the regulation of phosphate and calcium homeostasis. Human Klotho exists in both membrane bound and secreted forms, and is predominantly expressed in the kidney convoluted tubules, and to a lesser extent, in the brain, reproductive organs, endocrine glands, urinary bladder, skeletal muscle, placenta, and colon,. The full length transmembrane form has a large extracellular domain composed of two homologous subunits termed KL1 and KL2, which contain 516 and 439 amino acid residues, respectively, The predominant circulating form, which is derived from alternative RNA splicing, contains the KL1 subunit and constitutes the N-terminal sequence of transmembrane Klotho. A third Klotho protein of about 128 kDa has been identified in the blood and cerebrospinal fluid. This circulating protein arises from the action of an as yet unidentified protease which cleaves transmembrane Klotho just above and/or within the plasma membrane. Klotho has been shown to play a key role in the signaling cascade of fibroblast growth factor-23 (FGF-23), a bone derived hormone that acts in the kidney to inhibit phosphate reabsorption and vitamin D biosynthesis. Klotho promotes FGF-23 signaling through binding to FGFRI (IIIc) which converts this canonical FGF receptor into a specific receptor for FGF-23. In the absence of Klotho the function of FGF-23 is literally abolished.
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant Human Klotho is a 65-70 kDa glycoprotein containing 516 amino acid residues.
AA Sequence:
EPGDGAQTWA RFSRPPAPEA AGLFQGTFPD GFLWAVGSAA YQTEGG WQQH GKGASIWDTF THHPLAPPGD SRNASLPLGA PSPLQPATGD V ASDSYNNVF RDTEALRELG VTHYRFSISW ARVLPNGSAG VPNREGL RYY RRLLERLREL GVQPVVTLYH WDLPQRLQDA YGGWANRALA DH FRDYAELC FRHFGGQVKY WITIDNPYVV AWHGYATGRL APGIRGSP RL GYLVAHNLLL AHAKVWHLYN TSFRPTQGGQ VSIALSSHWI NPR RMTDHSI KECQKSLDFV LGWFAKPVFI DGDYPESMKN NLSSILPDF T ESEKKFIKGT ADFFALCFGP TLSFQLLDPH MKFRQLESPN LRQL LSWIDL EFNHPQIFIV ENGWFVSGTT KRDDAKYMYY LKKFIMETLK AIKLDGVDVI GYTAWSLMDG FEWHRGYSIR RGLFYVDFLS QDKML LPKSS ALFYQKLIEK NGFPPLPENQ PLEGTFPCDF AWGVVDNYIQ VSQLTKPISS LTKPYH
Biological activity:
Determined by the dose-dependent stimulation of the proliferation of murine NIH-3T3 cells. Recombinant human Klotho is effective in a concentration range of 0.5-2.0 μg/ml.
Format
Stabilizers:
None
Purity:
>98% by SDS-PAGE and HPLC
State:
Lyophilized purified protein
Purified
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