AR10140PU-S L-Asparaginase

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Product Description for L-Asparaginase

Presentation: Purified

Properties for L-Asparaginase

Product Category Proteins & Growth Factors
Quantity 0.5 kIU
Presentation Purified
Molecular weight 31731 Da
Source E. coli
Species (Protein) E. coli
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Swiss Prot Num:
Purity >96.0% as determined by: 
(a) Analysis by RP-HPLC. 
(b) Analysis by SDS-PAGE.

L-Asparaginase is an enzyme that depletes L-Asparagine "an important nutrient for cancer cells" resulting in cancer/tumor cell starvation. L-asparaginase is an anti-tumor agent derived from E.coli .,which can inhibit the growth of malignant cells. It is used mainly for the induction of remission in acute lymphoblastic leukaemia. Because of the lymph node origin of malignant B cells in Multiple Myeloma, L-Asparagine is an essential amino acid for their cell metabolism, and, consequently, L-Asparaginase may be of value in managing the disease.
The rationale behind asparaginase is that it takes advantage of the fact that ALL cellsare unable to synthesize the non-essential amino acidasparaginewhereas normal cells are able to make their own asparagine. These leukemic cells depend on circulating asparagine. Asparaginase however catalyzes the conversion of L-asparagine to aspartic acidand ammonia. This deprives the leukemic cell of circulating asparagine. 

Storage Store the antigen for 2 weeks at 2-8°C or at -20°C for longer. 
Avoid repeated freezing and thawing. 
Shelf life: One year from despatch.
L-Asparaginase produced from E.coli containing 303 amino acids and having a Molecular Mass of 31731 Dalton.
Biological activity:
One IU of L- Asparaginase is defined as that amount of enzyme required to generate 1 μmol of ammonia per minute at pH 7.3 and 
One unit of enzyme catalyzes hadrylazation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade.
Specific activity:
225 IU/mg
>96.0% as determined by: 
(a) Analysis by RP-HPLC. 
(b) Analysis by SDS-PAGE.
Sterile Filtered White lyophilized (freeze-dried) powder enzyme without additives.
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