Search for all "LALF"

Quick Overview



Product Description for LALF

Presentation: Purified

Properties for LALF

Product Category Proteins & Growth Factors
Quantity 0.1 mg
Presentation Purified
Species (Protein) Limulus
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 95.0% as determined by both RP-HPLC and SDS-PAGE analysis

LALF has been isolated from the membranes of amebocytes of Limulus polyphemus. LALF is a single-chain polypeptide with a molecular weight of 11,800.
The principal structure of the molecule consists of 102 amino acids and is partially homologous with structures of molecules in the lactalbumin-lysozyme family.
LALF has a high affinity binding for LPS and bactericidal for gram negative bacteria.
LALF binds the lipid A part of a variety of chemically diverse LPSs.
It can be used for endotoxin removal and detection. LALF reacts with specific antisera in ELISA and Western blots.

Storage Prior to reconstitution store at 2-8°C. 
Following reconstitution store undiluted at 2-8°C for one week or (in aliquots) at -20°C for longer. 
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). 
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Source P. pastoris
Recombinant LALF, entire coding region for Limulus antilipopolysaccharide factor derived from Pichia pastoris LALF is a 12,000 dalton protein.
The LALF is purified by proprietary chromatographic techniques.
Biological activity:
Antibacterial activity against E.coli less than 10ug/ml at 10e6 PFU/ml. Binds to and inactivates endotoxin (LPS).
Buffer System:
Lyophilized from a concentrated (1 mg/ml) solution containing no additives
> 95.0% as determined by both RP-HPLC and SDS-PAGE analysis
Sterile Filtered White lyophilized (freeze-dried) powder
Restore in sterile 20mM Acetic Acid not less than 100 μg/ml, which can then be further diluted to other aqueous solutions
  • LinkedIn