discontinued

AR10251PU-L Leptin Quadruple Antagonist

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1 mg / €980.00

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Leptin Quadruple Antagonist

AR10251PU-L

Product Description for Leptin Quadruple Antagonist

Leptin Quadruple Antagonist.
Presentation: Purified

Properties for Leptin Quadruple Antagonist

Product Category Proteins & Growth Factors
Quantity 1 mg
Presentation Purified
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity >98.0% as determined by both Gel filtration and SDS-PAGE
Add. information Protein content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.89 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Concentration 1.0 mg/ml (prior to lyophil.)
Storage

Prior to reconstitution store at RT for one month or desiccated below -18°C.
Upon reconstitution at > 0.1 Leptin mutant mg/ml and up to 2 mM and filter sterilization LEP mutant can be stored at 2-8°C or even RT for several weeks making it suitable for long term infusion studies using osmotic pumps.
At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Description
Description:
Recombinant Human Leptin Quadruple Mutant is a single polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a Mw of 16 kDa, Human Leptin was mutated, resulting in L39A/D40A/F41A/I42A.
Leptin Antagonist Quadruple Mutant was purified by proprietary chromatographic techniques.
AA Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Gln.
Biological activity:
Leptin Quadruple Antagonist Mutant is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transected with the long form of human Leptin receptor. It also inhibits various Leptin effects in several in vitro bioassays.
Format
Buffer System:
0.0045mM NaHCO3
Purity:
>98.0% as determined by both Gel filtration and SDS-PAGE
State:
White lyophilized (freeze-dried) powder
Purified
Reconstitution:
Restore in sterile 0.4% NaHCOadjusted to pH 8-9, not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
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