AR10251PU-S Leptin Quadruple Antagonist

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20 µg / €230.00

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Leptin Quadruple Antagonist


Product Description for Leptin Quadruple Antagonist

Leptin Quadruple Antagonist.
Presentation: Purified

Properties for Leptin Quadruple Antagonist

Product Category Proteins & Growth Factors
Quantity 20 µg
Presentation Purified
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity >98.0% as determined by both Gel filtration and SDS-PAGE
Add. information Protein content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.89 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Concentration 1.0 mg/ml (prior to lyophil.)

Prior to reconstitution store at RT for one month or desiccated below -18°C.
Upon reconstitution at > 0.1 Leptin mutant mg/ml and up to 2 mM and filter sterilization LEP mutant can be stored at 2-8°C or even RT for several weeks making it suitable for long term infusion studies using osmotic pumps.
At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Recombinant Human Leptin Quadruple Mutant is a single polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a Mw of 16 kDa, Human Leptin was mutated, resulting in L39A/D40A/F41A/I42A.
Leptin Antagonist Quadruple Mutant was purified by proprietary chromatographic techniques.
AA Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Gln.
Biological activity:
Leptin Quadruple Antagonist Mutant is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transected with the long form of human Leptin receptor. It also inhibits various Leptin effects in several in vitro bioassays.
Buffer System:
0.0045mM NaHCO3
>98.0% as determined by both Gel filtration and SDS-PAGE
White lyophilized (freeze-dried) powder
Restore in sterile 0.4% NaHCOadjusted to pH 8-9, not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
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