discontinued

AR10469PU-S Leptin Quadruple Antagonist

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20 µg / €230.00

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Leptin Quadruple Antagonist

AR10469PU-S

Product Description for Leptin Quadruple Antagonist

Leptin Quadruple Antagonist.
Presentation: Purified

Properties for Leptin Quadruple Antagonist

Product Category Proteins & Growth Factors
Quantity 20 µg
Presentation Purified
Source E. coli
Species (Protein) Sheep
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity >98.0% as determined by Gel filtration and SDS-PAGE analysis
Concentration 1.0 mg/ml (prior to lyophil.)
Storage Prior to reconstitution store at 2-8°C for one month or desiccated below -18°C.
Following reconstitution (at > 0.1 mg/ml and up to 2 mM and filter sterilization) store undiluted at 2-8°C or even at RT for one month making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. 
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant Ovine Leptin Antagonist Quadruple Mutant is a single non-glycosilated polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a Molecular Mass of ~ 16 kDa, Leptin was mutated, resulting in L39A/D40A/F41A/I42A mutant.
Purified by proprietary chromatographic techniques. 
Protein content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.21 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
AA Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Arg.
Biological activity:
Leptin Quadruple Antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of Ovine Leptin receptor. It also inhibits various leptin effects in several in vitro bioassays.
Format
Buffer System:
Lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3
Purity:
>98.0% as determined by Gel filtration and SDS-PAGE analysis
State:
White lyophilized (freeze-dried) powder
Purified
Reconstitution:
Restore in sterile 0.4% NaHCOadjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
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