PA1118X Leptin Quadruple Antagonist

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0.1 mg / €170.00

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Leptin Quadruple Antagonist


Product Description for Leptin Quadruple Antagonist

Leptin Quadruple Antagonist.
Presentation: Purified

Properties for Leptin Quadruple Antagonist

Product Category Proteins & Growth Factors
Quantity 0.1 mg
Presentation Purified
Molecular weight 16 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 98 % Greater than 98.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by SDS-PAGE.
Add. information Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.89 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Concentration 1,0 mg/ml
Storage Prior to reconstitution stable at RT for several weeks.
Following reconstitution (at > 0.1 Leptin mutant mg/ml and up to 2 mM) store the protein undiluted at 2-8°C for one week or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested.
Shelf life: one year from despatch.
Source E.coli
Leptin Quadruple Mutant Human Recombinant is a single polypeptide chain containing 146 amino and additional Ala at N-terminus acids, Human Leptin was mutated, resulting in L39A/D40A/F41A/I42A. Leptin Antagonist Quadruple Mutant was purified by proprietary chromatographic techniques.
AA Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Gln
Biological activity:
Leptin Quadruple Antagonist Mutant is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transected with the long form of human Leptin receptor. It also inhibits various Leptin effects in several in vitro bioassays.
Molecular weight:
16 kDa
Buffer System:
0.0045mM NaHCO3
>98% Greater than 98.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by SDS-PAGE.
Lyophilized purified protein (sterile filtered)
Restore in sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
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