AR10236PU-S Leptin Triple Antagonist

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Leptin Triple Antagonist


Product Description for Leptin Triple Antagonist

Leptin Triple Antagonist.
Presentation: Purified

Properties for Leptin Triple Antagonist

Product Category Proteins & Growth Factors
Quantity 20 µg
Presentation Purified
Molecular weight 16 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 98.0% as determined by both Gel filtration and SDS-PAGE analysis
Storage Prior to reconstitution store at RT for one month or desiccated below -18°C.
Upon reconstitution at > 0.1 mg/ml and up to 2 mM and filter sterilization Leptin mutant can be stored at 2-8°C or even RT for one month making it suitable for long term infusion studies using osmotic pumps.
At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Recombinant  Human Leptin Antagonist Triple Mutant is a single non-glycosilated polypeptide chain containing 146 amino and additional Ala at N-terminus acids, Leptin was mutated, resulting in L39A/D40A/F41A.
Recombinant Human Leptin Antagonist Triple Mutant was purified by proprietary chromatographic techniques.
AA Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Gln.
Biological activity:
Leptin triple antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. It also inhibits various leptin effects in several in vitro bioassays.
Buffer System:
Lyophilized from a concentrated (1 mg/ml) solution with 0.0045mM NaHCO3
> 98.0% as determined by both Gel filtration and SDS-PAGE analysis
White lyophilized (freeze-dried) powder
Restore in sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
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