discontinued

AR10428PU-S Leptin Triple Antagonist

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20 µg / €230.00

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Leptin Triple Antagonist

AR10428PU-S

Product Description for Leptin Triple Antagonist

Leptin Triple Antagonist.
Presentation: Purified

Properties for Leptin Triple Antagonist

Product Category Proteins & Growth Factors
Quantity 20 µg
Presentation Purified
Source E. coli
Species (Protein) Mouse
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 99.0% as determined by both Gel filtration and SDS-PAGE analysis.
Purification Method: Proprietary chromatographic techniques.
Concentration 0.65 mg/ml (prior to lyophil.)
Storage Prior to reconstitution store at 2-8°C for one month or desiccated below -18°C.
Following reconstitution (at > 0.1 mg/ml and up to 2 mM and filter sterilization) store undiluted at 2-8°C  or at RT for one month making it suitable for long term infusion studies using osmotic pumps. 
At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. 
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant Mouse Leptin Antagonist Triple Mutant is a single non-glycosilated polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a Molecular Mass of ~ 16 kDa, LEP was mutated, resulting in L39A/D40A/F41A mutant. 
Protein content: Protein quantization was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.21 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
AA Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Gln
Biological activity:
Capable of inhibiting Leptin-induced proliferation of BAF/3 cells stably transfected with the long form of Human Leptin Receptor. It also inhibits various Leptin effects in several in vitro bioassays.
Format
Buffer System:
Lyophilized from a concentrated (0.65 mg/ml) solution with 0.003mM NaHCO3
Purity:
> 99.0% as determined by both Gel filtration and SDS-PAGE analysis.
Purification Method: Proprietary chromatographic techniques.
State:
White lyophilized (freeze-dried) powder
Purified
Reconstitution:
Restore in sterile water or sterile 0.4% NaHCO3adjusted to pH 8-9, not less than 100 µg/ml, which can then be further diluted with other aqueous solutions.
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