AR10439PU-L Leptin Triple Antagonist

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Leptin Triple Antagonist


Product Description for Leptin Triple Antagonist

Leptin Triple Antagonist.
Presentation: Purified

Properties for Leptin Triple Antagonist

Product Category Proteins & Growth Factors
Quantity 1 mg
Presentation Purified
Source E. coli
Species (Protein) Rat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity >99.0% as determined by Gel filtration and SDS-PAGE analysis
Concentration 0.85 mg/ml (prior to lyophil.)
Storage Prior to reconstitution store at 2-8°C for one month or desiccated below -18°C.
Following reconstitution (at 0.1 mg/ml and up to 2mM and filter sterilization) store undiluted at 2-8°C or even RT for one month making it suitable for long term infusion studies using osmotic pumps.
At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Recombinant Rat Leptin Antagonist Triple Mutant is a singly non-glycosilated polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a Molecular Mass of ~16 kDa, Leptin was mutated, resulting in L39A/D40A/F41A mutant.
Recombinant Rat Leptin Antagonist Triple Mutant was purified by proprietary chromatographic techniques. 
Protein content: Protein quantization was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.21 as the extinction coefficient for a 0.1% (1 mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
AA Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Gln.
Biological activity:
Recombinant Rat Leptin Antagonist Triple Mutant is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of Human Leptin Receptor. It also inhibits various leptin effects in several in vitro bioassays.
Buffer System:
Lyophilized from a concentrated (0.85 mg/ml) solution with 0.003mM NaHCO3
>99.0% as determined by Gel filtration and SDS-PAGE analysis
White lyophilized (freeze-dried) powder
Restore in sterile water or sterile 0.4% NaHCOadjusted to pH 8-9, not less than 100 µg/ml, which can then be further diluted with other aqueous scroolutions.
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