discontinued

AR10471PU-L Leptin Triple Antagonist

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1 mg / €980.00

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Leptin Triple Antagonist

AR10471PU-L

Product Description for Leptin Triple Antagonist

Leptin Triple Antagonist.
Presentation: Purified

Properties for Leptin Triple Antagonist

Product Category Proteins & Growth Factors
Quantity 1 mg
Presentation Purified
Source E. coli
Species (Protein) Sheep
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity >98.0% as determined by both Gel filtration and SDS-PAGE analysis. 
Purification Method: Proprietary chromatographic techniques.
Concentration 0.65 mg/ml (prior to lyophil.)
Storage Prior to reconstitution store at 2-8°C for one month or desiccated below -18°C.
Following reconstitution (at 0.1 mg/ml and up to 2mM and Filter sterilization) store undiluted at 2-8°C 
At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. 
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant Ovine Leptin Antagonist Triple Mutant is a single non-glycosilated polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a Molecular Mass of ~ 16 kDa, Leptin was mutated, resulting in L39A/D40A/F41A mutant. 
Protein content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.21 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
AA Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Arg.
Biological activity:
Recombinant Ovine Leptin-Antagonist Triple Mutant is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of mouse leptin receptor. It also inhibits various leptin effects in several in vitro bioassays.
Format
Buffer System:
Lyophilized from a concentrated (0.65 mg/ml) solution with 0.003mM NaHCO3
Purity:
>98.0% as determined by both Gel filtration and SDS-PAGE analysis. 
Purification Method: Proprietary chromatographic techniques.
State:
White lyophilized (freeze-dried) powder
Purified
Reconstitution:
Restore in sterile water or sterile 0.4% NaHCO3adjusted to pH 8, not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
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