discontinued

AR10471PU-N Leptin Triple Antagonist

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0.1 mg / €300.00

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Leptin Triple Antagonist

AR10471PU-N

Product Description for Leptin Triple Antagonist

Leptin Triple Antagonist.
Presentation: Purified

Properties for Leptin Triple Antagonist

Product Category Proteins & Growth Factors
Quantity 0.1 mg
Presentation Purified
Source E. coli
Species (Protein) Sheep
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity >98.0% as determined by both Gel filtration and SDS-PAGE analysis. 
Purification Method: Proprietary chromatographic techniques.
Concentration 0.65 mg/ml (prior to lyophil.)
Storage Prior to reconstitution store at 2-8°C for one month or desiccated below -18°C.
Following reconstitution (at 0.1 mg/ml and up to 2mM and Filter sterilization) store undiluted at 2-8°C 
At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. 
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant Ovine Leptin Antagonist Triple Mutant is a single non-glycosilated polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a Molecular Mass of ~ 16 kDa, Leptin was mutated, resulting in L39A/D40A/F41A mutant. 
Protein content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.21 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
AA Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Arg.
Biological activity:
Recombinant Ovine Leptin-Antagonist Triple Mutant is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of mouse leptin receptor. It also inhibits various leptin effects in several in vitro bioassays.
Format
Buffer System:
Lyophilized from a concentrated (0.65 mg/ml) solution with 0.003mM NaHCO3
Purity:
>98.0% as determined by both Gel filtration and SDS-PAGE analysis. 
Purification Method: Proprietary chromatographic techniques.
State:
White lyophilized (freeze-dried) powder
Purified
Reconstitution:
Restore in sterile water or sterile 0.4% NaHCO3adjusted to pH 8, not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
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