discontinued

PA1120XC Leptin Triple Antagonist

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1 mg / €790.00

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Leptin Triple Antagonist

PA1120XC

Product Description for Leptin Triple Antagonist

Leptin Triple Antagonist.
Presentation: Purified

Properties for Leptin Triple Antagonist

Product Category Proteins & Growth Factors
Quantity 1 mg
Presentation Purified
Molecular weight 16 kDa
Source E. coli
Species (Protein) Rat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 99 % pure as determined by:
(a) Gel filtration analysis.
(b) Analysis by SDS-PAGE.
Add. information Protein Content: Protein quantization was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.21 as the extinction coefficient for a 0.1% (1 mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
General Readings
  1. Yingzhong Y, Droma Y, Rili G, Kubo K. Regulation of body weight by leptin, with special reference to hypoxia-induced regulation. Intern Med. 2006;45(16):941-6. Epub 2006 Sep 15. PubMed PMID: 16974055.
  2. Expression of endothelin-1 and adrenomedullin was not altered by leptin or resistin in bovine brain microvascular endothelial cells. Hypertens Res 2006 Jun;29(6):443-8
  3. Brennan AM, Mantzoros CS. Drug Insight: the role of leptin in human physiology and pathophysiology--emerging clinical applications. Nat Clin Pract Endocrinol Metab. 2006 Jun;2(6):318-27. PubMed PMID: 16932309.
  4. Leptin, estrogens and cancer. Mini Rev Med Chem 2006 Aug;6(8):897-907
  5. Atabek ME, Kurtoglu S, Pirgon O. Possible effect of leptin on renal magnesium excretion in adolescent patients with type 1 diabetes. Pediatr Int. 2006 Aug;48(4):393-7. PubMed PMID: 16911085.
  6. Sorace M, Tripodi L, Tripodi A, Groppetti D, Cremonesi F. Leptin: pharmacological aspects in gynecology. Clin Exp Obstet Gynecol. 2006;33(2):113-6. PubMed PMID: 16903251.
Storage Store the lyophilized Leptin Antagonist Triple Mutant at RT for up to six weeks or desiccated below -20°C for longer.
Please avoid repeated freezing and thawing.
Shelf life: one year from despatch
Following reconstitution at > 0.1 mg/ml and filter sterilization the protein can be stored at 2-8°C or even room temperature for several weeks, making it suitable for long-term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested.
Source Escherichia coli
Description
Description:
Recombinant Rat Leptin Antagonist Triple Mutant is a singly non-glycosilated polypeptide chain containing 146 amino and additional Ala at N-terminus acids. Leptin was mutated, resulting in L39A/D40A/F41A mutant. The product was purified by proprietary chromatographic techniques.
AA Sequence:
The sequence of the first five N-terminal was determined as Ala-Val-Pro-Ile-Gln.
Biological activity:
Recombinant Rat Leptin Antagonist Triple Mutant is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. It also inhibits various leptin effects in several i>in vitro bioassays.
Format
Buffer System:
The protein was lyophilized from a concentrated (0.85 mg/ml) solution with 0.003mM NaHCO3.
Endotoxin level:
< 0.1 ng/µg (IEU/µg).
Purity:
>99% pure as determined by:
(a) Gel filtration analysis.
(b) Analysis by SDS-PAGE.
State:
Lyophilized (sterile filtered) white freeze-dried powder.
Purified
Reconstitution:
Restore in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100 µg/ml, which can then be further diluted with other aqueous solutions.
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