PA1121XC Leptin Triple Antagonist

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1 mg / €790.00

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Leptin Triple Antagonist


Product Description for Leptin Triple Antagonist

Leptin Triple Antagonist.
Presentation: Purified

Properties for Leptin Triple Antagonist

Product Category Proteins & Growth Factors
Quantity 1 mg
Presentation Purified
Molecular weight 16 kDa
Source E. coli
Species (Protein) Sheep
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 98 % Greater than 98.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by SDS-PAGE.
Add. information Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.21 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Storage Prior to reconstitution stable at RT for several weeks.
Following reconstitution store (at > 0.1 Lep-tA mutant mg/ml and up to 2 mM and filter sterilization) the protein undiluted at 2-8°C for one week or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested.
Shelf life: one year from despatch.
Source Escherichia coli
Leptin Antagonist Triple Mutant Ovine Recombinant is a single non-glycosilated polypeptide chain containing 146 amino and additional Ala at N-terminus acids, Leptin was mutated, resulting in L39A/D40A/F41A mutant. Leptin Antagonist Triple Mutant Ovine Recombinant was purified by proprietary chromatographic techniques.
AA Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Arg.
Biological activity:
Leptin-Antagonist Triple Mutant Ovine Recombinant is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of mouse leptin receptor. It also inhibits various leptin effects in several in vitro bioassays.
Molecular weight:
~ 16 kDa
Buffer System:
0.003mM NaHCO3
>98% Greater than 98.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by SDS-PAGE.
Lyophilized purified protein
Restore in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
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