AR50387PU-S lldD (1-396, His-tag)

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20 µg / €300.00
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Quick Overview

lldD

Product Description for lldD

lldD.
Presentation: Purified

Properties for lldD

Product Category Proteins & Growth Factors
Quantity 20 µg
Synonyms ECK3595, JW3580, L-lactate dehydrogenase, lct, lctD
Presentation Purified
Molecular weight 45.3 kDa
Source E. coli
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P33232
Purity > 90 % by SDS - PAGE
Background lldD, also known as L-lactate dehydrogenase, is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD).
Concentration 1mg/ml (determined by Bradford assay)
General Readings Ogura Y., et al. (2009) Proc. Natl. Acad. Sci. U.S.A. 106:17939-17944
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant E.coli lldD protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGSHMIISAA SDYRAAAQRI LPPFLFHYMD GGAYSEYTLR RNVEDLSEVA LRQRILKNMS DLSLETTLFN EKLSMPVALA PVGLCGMYAR RGEVQAAKAA DAHGIPFTLS TVSVCPIEEV APAIKRPMWF QLYVLRDRGF MRNALERAKA AGCSTLVFTV DMPTPGARYR DAHSGMSGPN AAMRRYLQAV THPQWAWDVG LNGRPHDLGN ISAYLGKPTG LEDYIGWLGN NFDPSISWKD LEWIRDFWDG PMVIKGILDP EDARDAVRFG ADGIVVSNHG GRQLDGVLSS ARALPAIADA VKGDIAILAD SGIRNGLDVV RMIALGADTV LLGRAFLYAL ATAGQAGVAN LLNLIEKEMK VAMTLTGAKS ISEITQDSLV QGLGKELPAA LAPMAKGNAA
Molecular weight:
(420aa), confirmed by MALDI-TOF
Format
Buffer System:
20 mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 1mM DTT
Purity:
>90% by SDS - PAGE
State:
Liquid purified protein
Purified
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