AR31092PU-N Lysobacter Enzymogenes Arg-C

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20 µg / €230.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Lysobacter Enzymogenes Arg-C

AR31092PU-N

Product Description for Lysobacter Enzymogenes Arg-C

Lysobacter Enzymogenes Arg-C.

Properties for Lysobacter Enzymogenes Arg-C

Product Category Proteins & Growth Factors
Quantity 20 µg
Synonyms ler
Molecular weight 26.8 kDa
Source High-5 Insect cells
Species (Protein) Rat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
O87544
Purity > 98 % by SDS-PAGE gel and HPLC analyses
Background Proteases (also called Proteolytic Enzymes, Peptidases, or Proteinases) are enzymes that hydrolyze the amide bonds within proteins or peptides. Most proteases act in a specific manner, hydrolyzing bonds at or adjacent to specific residues or a specific sequence of residues contained within the substrate protein or peptide. Proteases play an important role in most diseases and biological processes including prenatal and postnatal development, reproduction, signal transduction, the immune response, various autoimmune and degenerative diseases, and cancer. They are also an important research tool, frequently used in the analysis and production of proteins. Arg-C specifically cleaves at the carboxyl side of Arginine residues. Arg-C has a sulfhydryl requirement; it is activated by dithiothreitol, cysteine, or other sulfhydryl containing reagents. The presence of calcium ions is essential. The enzyme is inhibited by oxidizing agents and sulfhydryl reactants and by Co2+, Cu2+, Cd2+, and heavy metal ions. 
Storage

Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month 
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Description
Description:
Recombinant Lysobacter Enzymogenes Arg-C is a 26.8 kDa protease consisting of 252 amino acid residues including a C-terminal His-Tag.
AA Sequence:
GVGDIGSSDY CEKDIVCRVK PSAEFLSASK SVARMVFTPK TGYTGYCSGT LLNNSNSPKR QLFWSAAHCI STQKVANTLQ TYWLYDATGC DNDTLSDKAV TLTGGATLLH SHATRDTLLL ELKSAPPSGA YYAGWNSSAI ATKGTAIEGI HHPSGDLKKY SLGSVTALSS TIDGKKPLTK VAWTTGVTEG GSSGSGLFTI SSTSGYQLRG GLYGGTSYCS APSDPDYYSQ LDGVWSSIKT YFSPHHHHHH HH
Biological activity:
The reaction is measured as an increase in absorbance at 253 nm resulting from the hydrolysis of N-benzoyl-L-arginine ethyl ester (BAEE).
Format
Purity:
>98% by SDS-PAGE gel and HPLC analyses
State:
Lyophilized purified protein
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