discontinued

PA1157X Macrophage Chemotactic Protein-1 (MCP-1) (MCAF)

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10 µg / €170.00

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Macrophage Chemotactic Protein-1 (MCP-1) (MCAF)

Product Description for Macrophage Chemotactic Protein-1 (MCP-1) (MCAF)

Macrophage Chemotactic Protein-1 (MCP-1) (MCAF).
Presentation: Purified

Properties for Macrophage Chemotactic Protein-1 (MCP-1) (MCAF)

Product Category Proteins & Growth Factors
Quantity 10 µg
Synonyms CCL2, MCP1
Presentation Purified
Molecular weight 14 kDa
Source E. coli
Species (Protein) Mouse
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 99 % Greater than 99.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
Add. information Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm .
2. Analysis by RP-HPLC, using a calibrated solution of Murine JE(MCP-1) as a Reference Standard.
Concentration 1 mg/ml
Storage Lyophilized Murine JE(MCP-1) although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution Murine JE(MCP-1) should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Source E.coli
Description
Description:
Recombinant Murine JE(MCP-1) produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 125 amino acids.Murine JE(MCP-1) is purified by proprietary chromatographic techniques.
AA Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Asp-Ala-Val.
Biological activity:
Murine JE(MCP-1)is fully biologically active when compared to standard. The Biological activity is calculated by its ability to chemoattract Balb/C mouse spleen MNCs at 1.0-20.0 ng/ml.
Molecular weight: 13803 Dalton.
Format
Buffer System:
Murine JE(MCP-1) was lyophilized from a concentrated sterile solution containing no additives.
Dimers:
Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Endotoxin level:
Less than 0.1 ng/µg (IEU/µg) of Murine JE(MCP-1).
Purity:
>99% Greater than 99.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
State:
Sterile Filtered White lyophilized (freeze-dried) powder.
Purified
Reconstitution:
It is recommended to reconstitute the lyophilized Murine JE(MCP-1) in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
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