PA1158 Macrophage Chemotactic Protein-1 (MCP-1) (MCAF)

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2 µg / €110.00

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Macrophage Chemotactic Protein-1 (MCP-1) (MCAF)

Product Description for Macrophage Chemotactic Protein-1 (MCP-1) (MCAF)

Macrophage Chemotactic Protein-1 (MCP-1) (MCAF).
Presentation: Purified

Properties for Macrophage Chemotactic Protein-1 (MCP-1) (MCAF)

Product Category Proteins & Growth Factors
Quantity 2 µg
Synonyms CCL2, MCP1
Presentation Purified
Molecular weight 14 kDa
Source E. coli
Species (Protein) Rat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 95 % Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
Add. information Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm .
2. Analysis by RP-HPLC, using a calibrated solution of Rat MCP-1 as a Reference Standard.
Concentration 1 mg/ml
General Readings 1. Mitomycin C upregulates IL-8 and MCP-1 chemokine expression via mitogen-activated protein kinases in corneal fibroblasts. Invest Ophthalmol Vis Sci 2007 May;48(5):2009-16
2. Elevated ICAM-1 and MCP-1 plasma levels in subjects at high cardiovascular risk are diminished by atorvastatin treatment. Atorvastatin on Inflammatory Markers study: a substudy of Achieve Cholesterol Targets Fast with Atorvastatin Stratified Titration. Am Heart J 2007 May;153(5):881-8
3. Inhibition of migration of monocytes and granulocytes in vivo by the peptide corresponding to sequence 65-76 of monocyte chemotactic protein-1 (MCP-1).
Dokl Biochem Biophys 2006 Nov-Dec;411:339-41
4. Elevated white blood cell count, CRP and fibrinogen levels are not associated with increased anti-endothelial and anti-ox-LDL antibody, MCP-1, and RANTES levels in early onset occlusive carotid artery disease. Cytokine 2007 Jan;37(1):44-50
5. Coxiella burnetii stimulates production of RANTES and MCP-1 by mononuc lear cells: modulation by adhesion to endothelial cells and its implication in Q fever. Eur Cytokine Netw 2006 Dec;17(4):253-9
6. Changes in serum and ascitic monocyte chemotactic protein-1 (MCP-1) and IL-10 levels in cirrhotic patients with spontaneous bacterial peritonitis. J Interferon Cytokine Res 2007 Mar;27(3):227-30
Storage Recombinant Monocyte Chemotactic Protein-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution Monocyte Chemotactic Protein-1 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Source E.coli
Recombinant Rat MCP-1 produced in E.Coli is a non-glycosylated, Polypeptide chain containing 125 amino acids.Rat Monocyte Chemotactic Protein-1 is purified by proprietary chromatographic techniques.
AA Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Asp-Ala-Val.
Biological activity:
MCP-1 is fully biologically active when compared to standard. The specific activity as determined by the ability of Rat MCP-1 to chemoattract human peripheral blood at 10-100ng/ml.
Molecular weight: 14092 Dalton.
Buffer System:
Recombinant MCP-1 was lyophilized from a concentrated sterile solution containing no additives.
Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Endotoxin level:
Less than 0.1 ng/µg (IEU/µg) of Rat Monocyte Chemotactic Protein-1.
>95% Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
Sterile Filtered White lyophilized (freeze-dried) powder.
It is recommended to reconstitute the lyophilized Rat MCP-1 in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
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