PA1160 Macrophage Chemotactic Protein-3 (MCP-3)

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Macrophage Chemotactic Protein-3 (MCP-3)

Product Description for Macrophage Chemotactic Protein-3 (MCP-3)

Macrophage Chemotactic Protein-3 (MCP-3).
Presentation: Purified

Properties for Macrophage Chemotactic Protein-3 (MCP-3)

Product Category Proteins & Growth Factors
Quantity 2 µg
Synonyms CCL7, MCP3
Presentation Purified
Molecular weight 9 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 99 % Greater than 99.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
Add. information Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm .
2. Analysis by RP-HPLC, using a calibrated solution of Human MCP-2 as a Reference Standard.
Concentration 1 mg/ml
General Readings
  1. Tsou CL, Peters W, Si Y, Slaymaker S, Aslanian AM, Weisberg SP, et al. Critical roles for CCR2 and MCP-3 in monocyte mobilization from bone marrow and recruitment to inflammatory sites. J Clin Invest. 2007 Apr;117(4):902-9. Epub 2007 Mar 15. PubMed PMID: 17364026. (Free PMC Article available, 9 images available)
  2. McManus C, Berman JW, Brett FM, Staunton H, Farrell M, Brosnan CF. MCP-1, MCP-2 and MCP-3 expression in multiple sclerosis lesions: an immunohistochemical and in situ hybridization study. J Neuroimmunol. 1998 Jun 1;86(1):20-9. PubMed PMID: 9655469.
  3. Uguccioni M, Loetscher P, Forssmann U, Dewald B, Li H, Lima SH, et al. Monocyte chemotactic protein 4 (MCP-4), a novel structural and functional analogue of MCP-3 and eotaxin. J Exp Med. 1996 May 1;183(5):2379-84. PubMed PMID: 8642349. (Free PMC Article available)
  4. Proost P, Van Leuven P, Wuyts A, Ebberink R, Opdenakker G, Van Damme J. Chemical synthesis, purification and folding of the human monocyte chemotactic proteins MCP-2 and MCP-3 into biologically active chemokines. Cytokine. 1995 Feb;7(2):97-104. PubMed PMID: 7780043.
  5. Uguccioni M, D'Apuzzo M, Loetscher M, Dewald B, Baggiolini M. Actions of the chemotactic cytokines MCP-1, MCP-2, MCP-3, RANTES, MIP-1 alpha and MIP-1 beta on human monocytes. Eur J Immunol. 1995 Jan;25(1):64-8. PubMed PMID: 7531149.
  6. Opdenakker G, Froyen G, Fiten P, Proost P, Van Damme J. Human monocyte chemotactic protein-3 (MCP-3): molecular cloning of the cDNA and comparison with other chemokines. Biochem Biophys Res Commun. 1993 Mar 15;191(2):535-42. PubMed PMID: 8461011.
Storage Lyophilized Monocyte Chemotactic Protein-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution Monocyte Chemotactic Protein-3 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Source E.coli
Recombinant Human MCP-3 produced in E.Coli is a non-glycosylated, Polypeptide chain containing 76 amino acids.Human Monocyte Chemotactic Protein-3 is purified by proprietary chromatographic techniques.
AA Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Val-Gly-Ile.
Biological activity:
MCP-3 is fully biologically active when compared to standard. The specific activity as determined by the ability of MCP-3 to chemoattract human peripheral blood at 10-100ng/ml.
Molecular weight: 9011 Dalton.
Buffer System:
Recombinant Human Monocyte Chemotactic Protein-3 was lyophilized from a concentrated sterile solution containing no additives.
Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Endotoxin level:
Less than 0.1 ng/µg (IEU/µg) of recombinant Monocyte Chemotactic Protein-3.
>99% Greater than 99.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
Sterile Filtered White lyophilized (freeze-dried) powder.
It is recommended to reconstitute the lyophilized MCP-3 in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
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