PA1104 Macrophage Colony Stimulating Factor (M-CSF)

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Macrophage Colony Stimulating Factor (M-CSF)

Product Description for Macrophage Colony Stimulating Factor (M-CSF)

Macrophage Colony Stimulating Factor (M-CSF).
Presentation: Purified

Properties for Macrophage Colony Stimulating Factor (M-CSF)

Product Category Proteins & Growth Factors
Quantity 2 µg
Synonyms M-CSF
Presentation Purified
Molecular weight 37 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 95 % Proprietary chromatographic techniques, > 95.0 % as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Background Granulocyte/Macrophage Colony-Stimulating Factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
General Readings
  1. Tee MM, Tesch GH, Nikolic-Paterson DJ, Brown FG. Human peritoneal mesothelial cells isolated from spent dialysate fluid maintain contaminating macrophages via production of macrophage colony stimulating factor. Nephrology (Carlton). 2007 Apr;12(2):160-5. PubMed PMID: 17371340.
  2. ławicki S, Czygier M, Gacuta-Szumarska E, Knapp P, Szmitkowski M. [The plasma levels and diagnostic utility of granulocyte colony stimulating factor (G-CSF) and macrophage - colony stimulating factor (M-CSF) in ovarian cancer patients]. Pol Merkur Lekarski. 2006 Nov;21(125):465-8. PubMed PMID: 17345841.
  3. Kirma N, Hammes LS, Liu YG, Nair HB, Valente PT, Kumar S, et al. Elevated expression of the oncogene c-fms and its ligand, the macrophage colony-stimulating factor-1, in cervical cancer and the role of transforming growth factor-beta1 in inducing c-fms expression. Cancer Res. 2007 Mar 1;67(5):1918-26. PubMed PMID: 17332318.
  4. Liu CH, Chen LH. Promotion of recombinant macrophage colony stimulating factor production by dimethyl sulfoxide addition in Chinese hamster ovary cells. J Biosci Bioeng. 2007 Jan;103(1):45-9. PubMed PMID: 17298900.
  5. Serum levels of granulocyte colony-stimulating factor (G-CSF) and macrophage colony-stimulating factor (M-CSF) in pancreatic cancer patients. Clin Chem Lab Med 2007;45(1):30-4
  6. Yang PT, Kasai H, Xiao WG, Zhao LJ, He LM, Yamashita A, et al. Increased expression of macrophage colony-stimulating factor in ankylosing spondylitis and rheumatoid arthritis. Ann Rheum Dis. 2006 Dec;65(12):1671-2. PubMed PMID: 17105859. (Free PMC Article available, 1 images available)
Storage Stable at room temperature for one month, should be stored desiccated below -20 °C. Upon reconstitution MCSF should be stored at 2 - 8 °C for one week and for future use below -20 °C. For long term storage it is recommended to add a carrier protein (0.1 % HSA or BSA). Avoid freeze-thaw cycles.
Shelf life: one year from despatch.
Source E.coli
Macrophage Colony Stimulating Factor Human Recombinant produced in E.coli is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 159 amino acids.
AA Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Glu-Val-Ser.
Biological activity:
The ED50, calculated by the dose-dependant stimulation of the proliferation of murine M-NFS-60 indicator cells was found < 5 ng/ml, corresponding to a Specific Activity of 2 x 10e5 IU/mg.
Molecular weight:
36.8 KD.
Buffer System:
0.1 gr HSA and 0.6 gr Manntiol
>95% Proprietary chromatographic techniques, > 95.0 % as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions
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