PA1165X Macrophage Inflammatory Protein-1 alpha (MIP-1a)

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Macrophage Inflammatory Protein-1 alpha (MIP-1a)


Product Description for Macrophage Inflammatory Protein-1 alpha (MIP-1a)

Macrophage Inflammatory Protein-1 alpha (MIP-1a).
Presentation: Purified

Properties for Macrophage Inflammatory Protein-1 alpha (MIP-1a)

Product Category Proteins & Growth Factors
Quantity 20 µg
Synonyms CCL3, MIP1 alpha
Presentation Purified
Molecular weight 8 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 98 % Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
Add. information Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm .
2. Analysis by RP-HPLC, using a standard solution of MIP-1 as a Reference Standard.
Concentration 0.55 mg/ml
General Readings
  1. De Francesco MA, Poiesi C, Ricotta D, Manca N. HIV p17 reverses the anti-inflammatory activity of IL-4 on IL-15 stimulated monocytes and modulates their ability to secrete MIP-1 alpha. Virus Res. 2006 Jun;118(1-2):170-7. Epub 2006 Jan 19. PubMed PMID: 16427155.
  2. Nishi T, Maier CM, Hayashi T, Saito A, Chan PH. Superoxide dismutase 1 overexpression reduces MCP-1 and MIP-1 alpha expression after transient focal cerebral ischemia. J Cereb Blood Flow Metab. 2005 Oct;25(10):1312-24. PubMed PMID: 15829914.
  3. Brueckmann M, Hoffmann U, Dvortsak E, Lang S, Kaden JJ, Borggrefe M, et al. Drotrecogin alfa (activated) inhibits NF-kappa B activation and MIP-1-alpha release from isolated mononuclear cells of patients with severe sepsis. Inflamm Res. 2004 Oct;53(10):528-33. PubMed PMID: 15597147.
  4. Jennes W, Vereecken C, Fransen K, de Roo A, Kestens L. Disturbed secretory capacity for macrophage inflammatory protein (MIP)-1 alpha and MIP-1 beta in progressive HIV infection. AIDS Res Hum Retroviruses. 2004 Oct;20(10):1087-91. PubMed PMID: 15585099.
  5. Shi BB, Wang HJ, Ji ZG, Wang JT, Li HZ. [Clinical significance of RANTES and MIP-1 alpha in acute rejection episode in kidney transplantation]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2004 Feb;26(1):70-2. PubMed PMID: 15052779.
  6. MIP-1 alpha and myeloma bone disease. Cancer Treat Res 2004;118:83-100
Storage Lyophilized rHuMIP-1α although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution rHuMIP-1α should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Source E.coli
Recombinant Human MIP-1 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids.The rHuMIP-1α is purified by proprietary chromatographic techniques.
AA Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be, Ala-Ser-Leu-Ala-Ala.
Biological activity:
UV spectroscopy at 280 nm using the absorbency value of 0.9 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Molecular weight: 7820 Dalton.
Buffer System:
Lyophilized from a concentrated solution in water containing no additives.
Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Endotoxin level:
Less than 0.1 ng/µg (IEU/µg) of rHuMIP-1.
>98% Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
Sterile Filtered White lyophilized (freeze-dried) powder.
It is recommended to reconstitute the lyophilized rHuMIP-1α in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
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