PA1169 Macrophage Inflammatory Protein-3 beta (MIP-3 beta) (Exodus-3, ELC)

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Macrophage Inflammatory Protein-3 beta (MIP-3 beta) (Exodus-3, ELC)

Product Description for Macrophage Inflammatory Protein-3 beta (MIP-3 beta) (Exodus-3, ELC)

Macrophage Inflammatory Protein-3 beta (MIP-3 beta) (Exodus-3, ELC).
Presentation: Purified

Properties for Macrophage Inflammatory Protein-3 beta (MIP-3 beta) (Exodus-3, ELC)

Product Category Proteins & Growth Factors
Quantity 5 µg
Synonyms CCL19, MIP3 beta
Presentation Purified
Molecular weight 9 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 99 % Greater than 99.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
Add. information Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm .
2. Analysis by RP-HPLC, using a standard solution of MIP-3beta as a Reference Standard.
Concentration 1.0 mg/ml
General Readings
  1. Hansson M, Lundgren A, Elgbratt K, Quiding-Järbrink M, Svennerholm AM, Johansson EL. Dendritic cells express CCR7 and migrate in response to CCL19 (MIP-3beta) after exposure to Helicobacter pylori. Microbes Infect. 2006 Mar;8(3):841-50. Epub 2006 Jan 13. PubMed PMID: 16500130.
  2. Pilkington KR, Clark-Lewis I, McColl SR. Inhibition of generation of cytotoxic T lymphocyte activity by a CCL19/macrophage inflammatory protein (MIP)-3beta antagonist. J Biol Chem. 2004 Sep 24;279(39):40276-82. Epub 2004 Jul 1. PubMed PMID: 15231820.
  3. Akahoshi T, Sasahara T, Namai R, Matsui T, Watabe H, Kitasato H, et al. Production of macrophage inflammatory protein 3alpha (MIP-3alpha) (CCL20) and MIP-3beta (CCL19) by human peripheral blood neutrophils in response to microbial pathogens. Infect Immun. 2003 Jan;71(1):524-6. PubMed PMID: 12496204. (Free PMC Article available, 3 images available)
  4. Townson JR, Nibbs RJ. Characterization of mouse CCX-CKR, a receptor for the lymphocyte-attracting chemokines TECK/mCCL25, SLC/mCCL21 and MIP-3beta/mCCL19: comparison to human CCX-CKR. Eur J Immunol. 2002 May;32(5):1230-41. PubMed PMID: 11981810.
  5. Parlato S, Santini SM, Lapenta C, Di Pucchio T, Logozzi M, Spada M, et al. Expression of CCR-7, MIP-3beta, and Th-1 chemokines in type I IFN-induced monocyte-derived dendritic cells: importance for the rapid acquisition of potent migratory and functional activities. Blood. 2001 Nov 15;98(10):3022-9. PubMed PMID: 11698286.
  6. Scapini P, Laudanna C, Pinardi C, Allavena P, Mantovani A, Sozzani S, et al. Neutrophils produce biologically active macrophage inflammatory protein-3alpha (MIP-3alpha)/CCL20 and MIP-3beta/CCL19. Eur J Immunol. 2001 Jul;31(7):1981-8. PubMed PMID: 11449350.
Storage Lyophilized MIP-3beta although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution MIP-3b should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Source E.coli
Recombinant Human MIP-3b produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 77 amino acids.Recombinant MIP-3 beta is purified by proprietary chromatographic techniques.
AA Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be, Gly-Thr-Asn-Asp-Ala.
Biological activity:
This macrophage inflammatory protein 3beta is fully biologically active when compared to standard. The Activity is calculated by the ability to chemoattract Human T cells using a concentration of 10-50 ng/ml.
Molecular weight: 8809 Dalton.
Buffer System:
Recombinant macrophage inflammatory protein 3beta, lyophilized from a concentrated solution in water containing no additives.
Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Endotoxin level:
Less than 0.1 ng/µg (IEU/µg) of MIP-3b.
>99% Greater than 99.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
Sterile Filtered White lyophilized (freeze-dried) powder.
It is recommended to reconstitute the lyophilized MIP-3b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
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